哺乳动物线粒体dna本地序列库的构建与环状可视化的研究计算机应用技术专业论文.docxVIP

内蒙古师范大学硕士学位论文ABSTRACT 内蒙古师范大学硕士学位论文 ABSTRACT The substance of the Bioinformatics is storage，retrieval and analysis of the DNA Sequence by computer．It is one of the advantage domains in life science and natural science．In recently，the number of sequenced genome is showing a dramatic rise in many countries，with the in—depth research on different species sequence．In this situation，there have been more pertinence demands for sequence analysis software or system which requested by the Bioinformatics researchers．．Such．as visualization of sequence structure， sequence analysis on off-line environment，creating of。the proprietary sequence database，Friendliness and usability of the alignment program，and SO on．All these fields have became the researcher’S focus by recently． Based on the research of the Bioinformatics knowledge，algorithm of the sequence alignment，methods of creating proprietary sequence database and development of the Bioinformatics software，we designed a way which was aimed at creating the local DNA sequence database of mitochondria of mammal，and then we designed a tool which was aimed at the visualization and the Function—·Domain—-Prediction of the loop molecular structure of mitochondrial DNA．The basic process is：first，we download the sample mitochondrial DNA sequence which has already finished the whole sequencing from the International Nucleotide Sequence Databank(GenBank、 EMBL、DDBJ)，and then we abstract the functional domain of every sample se 内蒙古师范大学硕士学位论文quences．Second，we 内蒙古师范大学硕士学位论文 quences．Second，we run the testing program to integrate all the sample se- quence files into one database file which is‘．fasta’form，then we called the BLAST modules￡from NCBI by WinSDK，and achieved the comparison of homology between the search-sequence and the local database．At last，we could forecast the region of functional domain of search—sequence according to the sequence which has the highest homology score．Experiment results show that this method could meet the requ