实时心肌声学造影定量评价缺血后适应对微循环的影响.pdfVIP

英文摘要 实时心肌声学造影定量评价缺血后适应对微循环的影响 Quantitative Evaluation of The Effects of Ischemic Postconditioning on Myocardial Microcirculation With Real-time Myocardial Contrast Echocardiography Abstract Objective: To evaluate the feasibility of real-time myocardial contrast echocardiography performing on models of myocardial ischemic-reperfusion rabbits, and to observe the effects of ischemic postconditioning on myocardial no-reflow. Methods: 60 New Zealand white rabbits were randomly divided into Sham operation(Sham) group, ischemia reperfusion(IR) group and ischemic postconditioning(IPostC) group, with 20 rabbits in each group. Sham group ：only thoracotomy without ligation, and suctures were inserted under the left anterior descending artery(LAD); IR group:LAD occlusion for 1h and reperfuseion for 2h; IPostC group :four episodes of 30s reperfusion and 30s occlusion after 1h ischemia, and before 2 hours of reperfusion. ECG monitoring was continuously performed during operation. And respectively we record ECG before ligating LAD, 10 minutes after ligating LAD and 2 hours after reperfusion. Blood was draw to evaluate serum troponin I, high sensitive C- reactive protein, SOD and MDA before operation, 2h after reperfusion and 1 week after the operation. Echocardiography and myocardial contrast echocardiography were performed and analyzed 1 week after the operation. Myocardial infarcted size of rabbits in each group was determined by staining with triphyltetrazolium chloride dye(TTC).Morphologic examination of myocardial tissue was done using optical microscope. Results: 1. 73 New Zealand white rabbits