rhG-CSF对大鼠脑缺血再灌注损伤后MMP-3和MMP-9表达的影响.pdfVIP

山西医科大学硕士学位论文 1 rhG-CSF ． 治疗脑缺血再灌注损伤大鼠，可改善早期神经功能缺损，促进肢体功能恢 复。 2．rhG-CSF治疗降低了脑缺血再灌注后MMP-3及MMP-9 的表达，并降低了血脑屏 障通透性，从而减轻脑水肿，这可能是rhG-CSF 的脑保护作用机制之一。 关键词 重组人粒细胞集落刺激因子；脑缺血再灌注；基质金属蛋白酶-3；基质金属蛋 白酶-9；血脑屏障 2 万方数据 山西医科大学硕士学位论文 TheeffectofrhG-CSFontheexpressionofMMP-3andMMP-9 proteinfollowingfocalcerebralischemia-reperfusioninjur inrats Abstract Objective Toexploretheneural protection mechanism of G-CSFb observingthe change of expression ofMMP-3 MMP-9 andthe permeabilit ofblood-brain barrier in SDrats with 、 cerebral ischemia-reperfusion injur that treated with recombinant human granulocyte colony-stimulatingfactor （rhG-CSF）. Methods Thirty-nine Sprague-Dawle male rats were randoml divided into 3 groups,sham-operated group,middle cerebral arter occlusion/reperfusion (MCAO/R)group and rhG-CSF treatment group,with 13 rats in each group . Establishing middle cerebral arter occlusionreperfusionratsmodelb longalineplugmethod.Dissectinginternalcarotidarter and externalcarotidarter insham-operatedgroup.TherhG-CSFtreatmentgroupreceivedadoseof 50ug/kg of rhG-CSF which was intraperitoneal injected at the onset of reperfusion after 2h occlusion and 24h after reperfusion.While sham- operated group and MCAO/R group were injected the same volume of saline.Longa 5-point scale was adopted to score neurological function at 48h after the reperfusion.The expression of MMP-3 and MMP-9 were detected b immunohistochemicalmethod.ThecontentofEvenblueinthebrainweredetectedb microplate reader. Transmission electronmicroscope was used to observe the ultrastructure ofblood-brain barrier.