电动流动分析系统以及毛细管电泳前处理技术的研究分析化学专业论文.docxVIP

AElfflPACTABSTRACT AElfflPACT ABSTRACT A novel mult-function flovv analysis system-．．．．electrokinetic flow analysis system (EKFA)was developed for simultaneous separation and pre—concentration of heterogeneous ion analytes with extremely low concentrations．m experimental technique and analytical application of EKFA system was discussed and the analytical conditions were optimized．Moreover,an integrative coupling technique of headspace liquid-phase microextraction(HS—LPME)and capillary zone chromatography(CZE) Was proposed and applied to the analysis of preservatives in food sample and soft drinks． (1)Determination of caffeic acid and gallic acid in tea samples by capillary electrophoresis with bi—directional electrostacking An analytical method combining bi—directional electrostacking with capillary electrophoresis Was proposed to analyze organic acid ions in tea samples．Salt bridges were replaced by porous cores to separate running buffer and electrode solution, which made the bi—directional electrostacking setup integratively．硼1e parameters were investigated,including electrostacking time，applied voltage，sample volume，etc． Under the optimal conditions，the enrichment factors of caffeic acid and gallic acid were 24 and 20，respectively．The limits of detection ranged from 0．2 to 0．4p egmL． (2)Determination of benzoic acid and sorbic acid in food sample and soft“11l(by integrative headspace liquid—phase microexlraction and capillary zone chromatography An integrative coupling technique of headspace liquid—phase microextraction (HS-LPME)and capillary zoRe chromatography(CZE)Was proposed and applied to the analysis of benzoic acid and sorbic acid in food and soft drink samples．，11圮 analytical technique was performed with one separation capillary to fix the acceptor volume，hold the microextraction droplet，inject the concentrated analytes and separate the analytes by CZE．The HS—LPME Was carried out、^rith 6 mL sample solution containing O．25 g／mL NaCl in a 1 4一mL sample