草木樨中天然香豆素的提取分离研究化学工程专业论文.docxVIP

草木樨中天然香豆素的提取分离研究摘 草木樨中天然香豆素的提取分离研究 摘 要 本文对草木樨中的香豆素提取工艺进行了研究和优化，开发出一条成本低、 污染少、适于放大生产的工艺路线，从而为草木樨的综合利用提供了技术方案和 试验数据。 首先，对草木樨中的香豆素进行了分析方法的研究。与紫外分光光度法相比， 高效液相色谱法采用XDB．C18色谱柱，甲醇．水(65：35)为流动相，流速 1．0mL／min，分析结果更加快速、准确。并对不同产地的香豆素含量进行分析， 来自银川地区草木樨花叶中香豆素的含量最高，达到0．664％，为提取纯化提供 了有效的数据。 其次，对草木樨中的香豆素进行了提取方法的研究。采用碱溶酸沉法对草木 樨中的香豆素进行提取，其提取物中香豆素含量过低，并且结构明显发生了改变。 采用水蒸气蒸馏法对草木樨中的香豆素进行提取，其提取物中香豆素的含量不到 2％，并伴有大量的焦油产生。 再次，重点研究了系统溶剂法提取草木樨中的香豆素。通过对提取工艺进行 探索后，确定了乙醇提取、石油醚脱色、氯仿萃取的工艺路线。经过单因素和正 交试验优化整个提取条件：在50℃下用80％乙醇回流提取3次，后用石油醚萃 取脱色3次，再经氯仿萃取1次。所得到的得粗提物，其收率约0．5％，香豆素 含量在20％左右。 最后，经过对不同大孔吸附树脂的筛选，选取LSA-40型大孔吸附树脂对香 豆素进行纯化，对树脂的相关因素进行研究，得到最优的柱层析纯化条件。经纯 化后所得提取物，含量最高达到78．9％。并对其吸附动力学进行研究，结果表明 吸附过程符合Freundlich等温吸附模型，并确定液膜扩散为吸附过程的控制步骤。 关键词：草木樨，香豆素，提取，大孔吸附树脂，高效液相色谱 Study Study on Extraction and Isolation Technology of Natural Coumarin frq ns Abstract In this paper,the extraction process of coumarin from melilotus was researched and optimized，and the process was developed，which not only Was low cost and less pollution，but also Was suitable for magnified production．The objective of research Was to provided scheme and experiment data for utilization of melilotus． Firstly,analysis method of coumarin in melilotus Was researched．Compared with UV spectrophotometry，HPLC with XDB—Ct s colunm,mobile phase of methanol-water(65：35)and flow rate of 1．0mL／min Was rapid and accurate．The content of coumarin from different areas was analysed，and the content of coumarin from flower and leaf of Ym Chuan Was 0．664％，which Was the hi曲est，to provided available data for extraction and purification． Secondly,extraction method of coumarin from melilotus Was investigated． Coumarin in melilotus Was extracted in the method of acid-alkali，and the content of coumarin in product Was too low and its configuration Was changed in evidence． Coumarin in melilotus was extracted in the method of steam distillation，and the content of coumarin in product Was less than 2％and plentiful tar as the by-product Was found at the same time． Thirdly,extract