兔脑脊液中万古霉素LC-MSMS测定方法的建立及其与冰片.PDFVIP

兔脑脊液中万古霉素 LC-MS/MS 测定方法的建立及其与冰片合用的 药动学研究  李旭梅，褚正民，涂厉标(嘉兴市第二医院，浙江 嘉兴 314000) 摘要：目的 建立一种快速灵敏的 LC-MS/MS 用于兔脑脊液中万古霉素的测定，并进行万古霉素的药动学研究。方法 观 察兔分别单独静脉注射万古霉素以及先灌胃给予冰片再静脉注射给予万古霉素后脑脊液中万古霉素的浓度变化。采用内 标法进行定量分析，获得脑脊液中万古霉素的浓度，并采用 DAS 药动学软件拟合主要动力学参数 AUC 、Cmax 、T1/2 等。 结果 脑脊液中万古霉素的批间精密度为 4.7%~6.1% ，批内精密度为 1.4%~7.1%，准确度为 93.6%~106.0%。万古霉素的 线性范围为 50~5 000 ng·mL1 ，标准曲线的相关系数 r0.99 。合用冰片组兔脑脊液中万古霉素的 AUC 和 Cmax 值明显高于 单用万古霉素组。结论 该方法适用于万古霉素在兔脑脊液中的药动学研究。冰片可以提高万古霉素透过血脑屏障的能力。 关键词：万古霉素；冰片；血脑屏障；药动学 中图分类号：R969.1 文献标志码：B 文章编号：1007-7693(2019)03-0281-05 DOI: 10.13748/ki.issn1007-7693.2019.03.005 引用本文：李旭梅, 褚正民, 涂厉标. 兔脑脊液中万古霉素 LC-MS/MS 测定方法的建立及其与冰片合用的药动学研究[J]. 中国现代应用药学, 2019, 36(3): 281-285. Development of LC-MS/MS for the Quantitation of Vancomycin in Rabbit Cerebrospinal Fluid and Its Application in Pharmacokinetic Study of Co-administrated with Borneol LI Xumei, ZHU Zhengmin, TU Libiao(The Second Hospital of Jiaxing, Jiaxing 314000, China) ABSTRACT: OBJECTIVE To develop a rapid, highly sensitive and selective performance LC-MS/MS for the determination of vancomycin in rabbit cerebrospinal fluid. Explore and complete the pharmacokinetic study of vancomycin in rabbits. METHODS The concentration of vancomycin in the cerebrospinal fluid was observed after intravenous injection of vancomycin in rabbits and intragastric administration of borneol followed by intravenous injection of vancomycin. Internal standard method was used in analysis to quantify the vancomycincerebrospinal fluid concentration. DAS software was used to obtain kinetic parameters such as AUC, Cmax , and T1/2. RESULTS The inter-day precision of vancomycin in rabbit cerebrospinal fluid was in the range of 4.7%6.1%, the intra-day precision was in the range of 1.4%7.1% and the accuracy was in the range of 93.6%106.0%, respectively. Linear calibrati