表面共价接枝肝素改善聚碳酸酯型聚氨酯血液相容性研究材料学专业论文.docxVIP

中文摘要为了改善聚碳酸酯聚氨酯的血液相容性，得到良好的抗凝血材料，本文主 中文摘要 为了改善聚碳酸酯聚氨酯的血液相容性，得到良好的抗凝血材料，本文主 要研究了三种聚氨酯膜表面接枝肝素方法，并用各种测试方法对改性后的聚合 物进行考察。 采用多步共价接枝法在聚碳酸酯型聚氨酯膜上接枝肝素，用甲苯胺蓝检测 法定量考察肝素降解后在膜上的接枝量。结果证实该方法接枝肝素量很少。 采用氨气低温等离子体处理法在聚碳酸酯型聚氨酯膜上接枝肝素，在聚乙 二醇接枝后的聚氨酯膜上用氨气等离子体处理，使膜表面产生氨基，再接枝肝 素。用甲苯胺蓝检测法定量考察肝素降解后在膜上的接枝量。结果证实该方法 接枝肝素量有所提高。 采用氨基封端的聚乙二醇在聚碳酸酯型聚氨酯膜上接枝肝素，首先利用 HDI活化聚氨酯膜，然后接枝氨基封端的聚乙二醇，最后再接枝肝素。考察了 众多反应工艺条件后确定最佳肝素接枝条件。通过甲苯胺蓝检测法定量考察肝 素降解后在膜上的接枝量。经过IR(红外分析)、XPS(X射线光电子能谱)、 DSC(差示扫描量热法)、接触角、吸水率分析改性前后聚氨酯特性的变化，用 AFM、SEM评价改性前后膜的表面形态的变化。．血小板粘附实验考查了改性的 聚氨酯膜的血液相容性。结果证实了有一定量的肝素接枝到聚氨酯膜表面，且 血液相容性测试证明了改性后，材料表面血小板吸附很少，血液相容性优异。 关键词：血液相容性，聚碳酸酯聚氨酯，肝素，表面改性 I ’ ABSTRACTIn ABSTRACT In order to improve blood compatibility of polycarbonate urethane，three different methods attaching heparin to the surface of polycarbonate urethane(PCU)were investigated and the modified polymers were characterized by variety methods． Multi—step covalent method was used to attach heparin to PCU surface，to determining the amount of heparin immobilized on PCU surface by toluidine blue colorimetric method，but the heparin grafting density was little． NH3 plasma method was used to attach heparin to the PCU surface．The PEG grafted PCU film with ammonia plasma treatment，to introduce the amino groups onto the PCU film surface，then grafting heparin．To determining the amount of heparin immobilized on PCU surface by toluidine blue colorimetric method，and the heparin grafting density Was increased． Amino·PEG method was also used to attach hepadn tO the PCU surface by a three·step procedure．First,the PCU film was treated with hexamethylene diisocyanate(HDI)in toluene．Second，the Amino-PEG(Mn=2000)was reacted with isocyanate groups linking on the surface of PCU film to obtain the PCU·APEG film． Third，the PCU·APEG was coupled with heparin．The optimal hepadn graft conditions were determined by examining lots of reaction process conditions．The amount of heparin covalently immobilized on the PCU-APEG membrane was determined by toluidine blue method．The modified