口腔扁平苔藓患者外周血microRNA-155及-第三军医大学学报.DOCVIP

201411057 论著 DOI：10.16016/j.1000-5404.201411057 慢性牙周炎患者唾液中miR-146a的表达及临床意义 赵家珍，闫文娟，吴补领（510515广州，南方医科大学南方医院口腔科） [摘要]目的 通过检测慢性牙周炎患者唾液中miR-146a的表达，以探讨miR-146a在慢性牙周炎发病中的作用及意义。方法 收集2012年1月至2013年8月期间南方医院口腔科收治的48例慢性牙周炎患者（其中男性28例，女性20例，平均年龄42岁）。同时选择20名性别、年龄匹配的健康志愿者作为对照组。采用实时荧光定量PCR技术检测上述人员唾液上清中miR-146a的表达水平。对治疗组患者（32例）进行牙周基础治疗，分别记录治疗前和治疗6周后患者的探诊深度（probing depth，PD）、临床附着丧失（attachment loss，AL）及菌斑指数（plaque index，PLI），并检测治疗后唾液上清液标本中miR-146a的表达。结果 慢性牙周炎患者唾液上清液中miR-146a的表达水平（2.52±0.87）显著高于健康对照组（0.81±0.58）（P＜0.01）。治疗组患者经过治疗后的牙周临床指数、唾液中miR-146a表达水平均显著降低（P＜0.05）。唾液中miR-146a水平与PD（r=0.574， P＜0.05)、AL（r=0.629，P＜0.01)及PLI（r=0.758， P＜0.01)均呈正相关。结论 慢性牙周炎患者唾液上清中miR-146a表达水平明显高于正常人，并在牙周基础治疗后显著下降，提示唾液miR-146可作为牙周病防治及疗效评估的潜在靶分子。 [关键词]慢性牙周炎；唾液；微小RNA；miR-146a Expression of clinical value of saliva miR-146a in patients with chronic periodontitis Zhao Jiazhen， Yan Wenjuan， Wu Buling（Southern Medical University；Dept. of Stomatology，Nanfang Hospital，Southern Medical University， Guagnzhou， 510515，China） [Abstract] Objective To examine miR-146a saliva expression in patients with chronic periodontitis and explore its clinical value. Methods Forty eight chronic periodontitis patients who were admitted into the First Affiliated Hospital of Nanchang University from January 2012 to August 2013 were investigated (including 28 male and 20 female with mean age of 42 years). Twenty age and sex matched healthy volunteers were enrolled as healthy controls. 32 chronic periodontitis patients were treated with initial therapy for 6 weeks. Fresh saliva samples were collected before and after the treatment， and probing depth (PD)， attachment loss (AL) and plaque index (PLI) were recorded. Real-time PCR was performed to detect the expression of saliva miR-146a. Results The expression of saliva miR-146a were higher in chronic periodontitis patients (2.52±0.87) than those in the healthy controls (0.81±0.58) (P＜0.01). After initial therapy， all clinical parameters improved. MiR-146a expression was reduced a