应用动物活体生物发光技术观察紫杉醇混合胶束的抑瘤效果-药学学报.PDFVIP

530 药学学报 Acta Pharmaceutica Sinica 2010, 45 (4) : 530534 研究简报 应用动物活体生物发光技术观察紫杉醇混合胶束的抑瘤效果 1 1 1 1 2 1* 1* 文 , 孙敏敏 , 刘 楠 , 黄志起 , 聂尚海 , 郝艳丽 , 张小宁 ( 1. 清华大学医学院, 北京 100084; 2. 北京东胜创新生物科技有限公司, 北京 100085) 关键词: 活体动物内光学成像; 生物发光; 荧光素酶; 混合胶束; 紫杉醇 中图分类号: R943 文献标识码:A 文章编号: 05 13-4870 (2010) 04-0530-05 Study on the anti-tumor effect of paclitaxel mixed micelle by using in vivo optical imaging technique 1 1 1 1 2 1* 1* TAI Wen , SUN Min-min , LIU Nan , HUANG Zhi-qi , NIE Shang-hai , HAO Yan-li , ZHANG Xiao-ning (1. Medical School of Tsinghua University, Beij ing 100084, China; 2. Eastwin Lif e Sciences Inc., Beij ing 100085, China) Abstract: In vivo tumor imaging technique method based on bioluminescence principle was established to evaluate the anti-tumor effect of paclitaxel mixed micelle (PMM). MDA-MB-231 tumor cells with luciferase reporter vectors were firstly implanted into nude mice, and subsequently the luciferase substrate was regularly inj ected during intraperitoneal administration of PMM. Then the tumor size, growth and the intensity of light signals were monitored with in vivo imaging technique. The method of luciferase tumor in vivo imaging could be real-time, reliable and exact in labeling and reflecting the growth of tumors, and the observed results were consistent with that by conventional method, so it would be a feasible approach to study anti-tumor effect of drugs. The anti-tumor effect of paclitaxel mixed micelle was observed by this method, and the results showed that