基于DNA-纳米金探针技术检测环境内分泌干扰物-分析化学专业论文.docxVIP

聊城大学硕士学位论文 聊城大学硕士学位论文 聊城大学硕士学位论文 聊城大学硕士学位论文 PAGE PAGE iv iii iii (I0-I) = 402.0 lg CBPA+4534.5，相关系数 R2 为 0.9929，检出限为 0.07 pg/mL。所建立 的方法用于不同温度下食品包装袋浸出液中双酚 A 含量的测定，结果满意。 二、双标记纳米金放大时间分辨荧光免疫分析测定双酚 A 采用双酚 A 完全抗原包被微孔板，样品中双酚 A 半抗原与固相抗原竞争结合双 标记纳米金上的双酚 A 抗体，然后双标记纳米金上 DNA 链另一端修饰的生物素与铕 螯合物标记的链霉亲和素相结合，以纳米金为载体放大荧光信号，建立了双标记纳米 金放大时间分辨荧光免疫分析测定双酚 A 的新方法。在优化的条件下，该方法在 1.0×10-6-1.0 ng/mL 范围内的线性关系良好，线性回归方程：ΔI (I0-I) = 784.0 lg CBPA + 6572.7，相关系数 R2 为 0.9925，检出限为 0.3 fg/mL。将此方法用于瓶装水中双酚 A 的测定，结果满意。 三、双标记纳米金放大时间分辨荧光免疫分析测定己烯雌酚 基于固相抗原竞争反应模式，采用己烯雌酚完全抗原包被微孔板，固相抗原与样 品中的己烯雌酚半抗原竞争结合双标记纳米金上的己烯雌酚抗体，将纳米金作为信号 放大工具，建立了包被抗原竞争免疫反应模式的双标记纳米金放大时间分辨荧光免疫 分析测定己烯雌酚的新方法。在优化的条件下，该方法在 1.0×10-6-10.0 ng/mL 之间有 良好的线性关系，其线性回归方程为 ΔI = 690.8 lg CDES + 6198.3，相关系数 R2 为 0.9989，检出限为 0.4 fg/mL。该方法用于牛奶中己烯雌酚含量的测定，结果令人满意。 关键词：铕；荧光免疫分析；时间分辨荧光免疫分析；纳米金；双标记纳米金；双 酚 A；己烯雌酚 ABSTRACT The environmental endocrine disrupting Chemicals (EDCs) were also called environmental hormone. Most EDCs have stable chemical properties. They can through various channels to enter the body, and then they can interfere with the natural hormones in the body. They can influence nerve system, reproductive system and immune system, and would affect the balance and development of the body oneself, caused variety diseases, to harm human health, and even will harm childrens growth. EDCs and its harm have become the focus of the world. Currently, more than 80 kinds of environmental endocrine disruptors were found and confirmed in daily life. Its detection method inclued chromatography method, chemiluminescence analysis, electrochemical analysis, and immune analysis, etc. Immune analysis method has fast analysis speed, simple, easy, high specificity, high selectivity, etc. And it is especially suitable for the analysis of biological samples, but the sensitivity is still to be improved. Because of the huge reactive surface area and biocompatible of gold nanoparticles, it can combine many DNA and protein. The DNA on the nanoparticles can exponential amplificati