茶多酚葡萄柚提取物联合应用对变异链球菌生长产酸的影响.DOC

PAGE 2 PAGE 3 编号 201009024 刊用形式 论著 PAGE 1 —— —— —— —— —— —— —— [基金项目] 福建省医学创新课题（2009-CXB-36） [通信作者] 黄晓晶，电话：（0591E-mail： HYPERLINK mailto:hxiaoj@163.com hxiaoj@163.com 茶多酚与仅仅是建议加入，但不加也可以。葡萄柚提取物联合应用对变异链球菌生长、产酸的影响 仅仅是建议加入，但不加也可以。 黄晓晶，卢国英，刘建党，江 山，雷丽珊（350002福州，福建医科大学附属口腔医院牙体牙髓科） [摘要] 目的 探讨茶多酚与葡萄柚提取物联合应用对变异链球菌生长、产酸的影响。方法 采用棋盘稀释法测定茶多酚葡萄柚提取物混合液对变异链球菌的最低抑菌浓度（MIC）值，计算分级抑制浓度(FIC)指数来判定二者的联合效应；并测定低于MIC浓度的茶多酚葡萄柚提取物混合液对变异链球菌产酸的影响。结果 茶多酚和GSE单独用药时，其MIC分别为2 mg/ml和46.88 mg/L，联用时可降低单组分的使用浓度，如茶多酚1 mg/ml联用GSE 37.5 mg/L即可达到联合应用的MIC，FIC=0.75。结论 茶多酚与葡萄柚提取物联合应用对变异链球菌的体外抗菌活性具有相加作用，较各自的单组份用药在更低浓度即可有效抑制变异链球菌的生长、产酸。 [关键词] 茶多酚；葡萄柚提取物；变异链球菌；抗菌活性；棋盘法 [中图法分类号] R781.1 [文献标识码] A Effect of combined application of tea polyphenols with and grapefruit -seed extract on growth and acid production of Streptococcus mutans Huang Xiaojing, Lu Guoying, Liu Jiandang, Jiang Shan, Lei Lishan (School of Stomatology, Fujian Medical University, Fuzhou 350002, [Abstract] Objective To investigate the effect of combined application of Ttea pPolyphenols (TPs) and gGrapefruit -seed extract (GSE) on the growth and acid generation production of Streptococcus mutans. Methods Minimal inhibitory concentration (MIC) of combined applicationmixed solution of TPs andwith GSE on the growth of Streptococcus mutans ATCC 25175 was measured with by the Ccheckerboard method. Fractional inhibitory concentration (FIC) was calculated accordingly to evaluate the combined effect of TPs and GSE. The effect of mixed solutions of TPs and GSE, with a concentrations lower than MIC, on the acid production of Streptococcus mutans ATCC 25175 of combined application of TP and GSE with concentration lower than MIC was also observed. Results Compared with the application of TPs (MIC = 2 mg/mL) or GSE (MIC = 46.88 mg/L) alone, Tthe combined application of TPs and GSE could lower the MICs of both components. For instance, TPs 1mg/mL comb