基于半夏泻心汤及其拆方治疗胃溃疡的实验研究探讨寒热并用配伍规律-方剂学专业论文.docxVIP

成都中医药大学博士毕业论文 成都中医药大学博士毕业论文 PAGE PAGE 10 结论：①半夏泻心汤及其拆方可显著改善胃溃疡大鼠的一般行为表现及胃 溃疡的炎症程度。②半夏泻心汤及其拆方对胃溃疡大鼠有明显的治疗作用，其作 用机制包括抑制炎性因子的释放；提高机体的抗氧化能力并减轻脂质过氧化损 伤；减轻胃酸对胃粘膜的侵蚀；协调胃肠运动；改善微循环；提高胃粘膜的修复 能力；上调内源性保护蛋白 HSP27、HSP70 mRNA 的表达。③半夏泻心汤方中 不同属性的药物从不同方面对胃溃疡发挥治疗作用。寒凉组药物在降低胃组织中 TNF-α、IL-8 含量等方面作用显著。温热组药物在升高血清中 NO 含量，降低血 清中 PAF、ET 含量等方面作用显著。补益组药物在升高胃粘膜 EGF、VEGF、 bFGF、PDGF 阳性表达方面作用显著。温补组药物升高胃组织中 SOD 含量，并 降低 MDA 含量，上调内源性保护蛋白 HSP27、HSP70 mRNA 的表达方面作用 显著。寒补组药物降低胃组织中 GAS、MTL 含量等方面作用显著。④寒热并用 配伍能消除单用寒凉或温热药的局限和不足，通过不同的途径和作用靶点来发挥 对胃溃疡的治疗作用，在很多方面产生了协同增效作用。 关键词： 胃溃疡 半夏泻心汤 寒热并用 拆方 炎性因子 修复因子 热休克蛋白 ABSTRACT Objective: Observe the influence on banxiaxiexin decoction and diffenent herbal formulation of component about gastric ulcer rat model in respects of inflammatory factors, repair factor and Hsp27, Hsp70 protein expression, and analysis the mechanism of action about the treatment effects of banxiaxiexin decoction and its Subdivisions, investigate the Significance of diffenent herbal formulation of component in banxiaxiexin decoction on treat gastric ulcer (GU), reveal the superiority and targets of compatibility of both cold and heat . Methods: Select male and female SD rats and establish the gastric ulcer animal model of mixed cold and heat syndromes. After 24 hours, all the rats except blank group and sham operation group were given of banxiaxiexin decoction and different herbal formulation of component, all rats were sacrificed after 28 days, observe rats’ pathological morphology change of gastric tissue, test SOD activity in xanthine oxidase method, gastric tissue MDA content in thiobarbituric acid method, serum NO Content in nitrate reductase method, gastric tissue TNF-α, IL-8, GAS, MTL and serum PAF, ET content in ELISA method, gastric mucosal EGF, VEGF, bFGF, PDGF expression in immunohistochemical detection, HSP27, HSP70mRNA expressions of gastric tissue of rats in RT-PCR method. Result: ①General behavior: The rats’ spirit, diet, fur color and wei