米曲霉果糖基转移酶催化合成蔗糖6乙酸酯.PDF

273 精 细 化 工 Vo.l 27, No. 3 2 010 3 FINE CHEM ICALS Mar. 2 0 1 0 6 毛多斌,刘国明,韩亚伟,杨雪鹏, 陈利平 ( , 450002) :(Asp erg illus oryzae)( s)6( g6a)6 ( s6a) m ( g6a)m ( s)pH , : 50 pH= 62m ( g6a)m ( s) = 13 48 h 40 g/L, g6a 2669% :; 6; ; : TQ033 : A : 1003- 5214( 2010) 03- 0234- 04 Synthesis of Sucrose6acetate Catalyzed by Fructosyltransferase fromA sp erg illus ory zae MAO Duobin, LIU Guoming,HAN Yawei, YANG Xuepeng, CHEN Liping (Zhengzhou University of Light Industry, School of Food and B iological Engineering, Zhengzhou 450002, enan, China) Abstract:The synthesis reaction of sucrose6acetate( s6a)w ith sucrose( s) and glucose6acetate( g 6a) catalyzed by fructosyltransferase fromA spergillus oryzae was investigated. The effects ofmass ratio of reactantsm ( g6a)m ( s), reaction temperature, reaction tmi e, pH, mass concentration of enzyme on the reaction were examined. The onefactor expermi ental results showed that the optmi um yield was obtained at 50 , pH = 62, m ( g6a) m ( s) = 13, reaction tmi e 48 h and mass concentration of enzyme40 g/L. On this condition themolar convert ratio of g6awas 2669%. Key ords:A sp ergillus oryzae; sucrose6acetate; fructosyltransferase; biological engineering Foundation item: Supported byNationalNaturalScience Foundation ofChina(ProjectNo. [6] Subtilis NCIB 11871 , 6( s6a) g6a s6a, , s6a 48% , s6a , ,: [ 1] [2] [ 3] , , ,