支气管肺发育不良新生鼠肺组织甲状腺转录因子及波形蛋白的表达意义.PDFVIP

2012;32(8) 南方医科大学学报（J South Med Univ ） ·1111 · 基础研究 支气管肺发育不良新生鼠肺组织甲状腺转录因子及波形蛋白的 表达意义 1 2 2 1 陈 坤 ，蒋 朴 ，邓世雄 ，王 念 1 2 重庆医科大学 附属第一医院，法医教研室，重庆 400014 摘要：目的 观察高氧暴露新生鼠肺泡Ⅱ型细胞（AEC Ⅱ）标记［甲状腺转录因子，（TTF- 1）］及成纤维细胞标记［波形蛋白， （Vimentin）］的表达规律，探讨支气管肺发育不良（BPD）发病机制。方法新生小鼠随机分为：对照组及BPD 组，高浓度氧暴露制 作BPD 小鼠模型，观察肺组织病理变化，于实验后3、7、14、21 d 应用免疫荧光双标及荧光定量PCR 技术检测TTF- 1及Vimentin 蛋白、mRNA 的表达情况。结果与对照组比较，BPD 组高氧暴露后小鼠肺组织逐渐出现肺泡发育障碍、胶原沉积明显；AEC Ⅱ 标记TTF- 1表达逐渐减弱，成纤维细胞标记Vimentin 表达逐渐增强，14、21 d 时两者可见明显的共表达；BPD 组21 d TTF- 1 mRNA 表达较同时间对照组下调（P0.05），而Vimentin mRNA 表达较同时间对照组上调（P0.05）。结论高氧致AEC Ⅱ向成纤 维细胞转化可能是BPD 纤维化的重要机制。 关键词：支气管肺发育不良；高氧症；肺泡Ⅱ型上皮细胞；分化 中图分类号：R722 文献标志码：A 文章编号：1673-4254（2012）08-1111-05 doi: 10.3969/j.issn. 1673-4254.2012.08.08 /kcms/detail/44.1627.R0933.001.html Expression of thyroid transcription factor-1 and vimentin in neonatal mice with bronchopulmonary dysplasis 1 2 2 1 CHEN Kun , JIANG Pu , DENG Shixiong , WANG Nian 1First Affiliated Hospital, 2Department of Forensic Medicine, Chongqing Medical University, Chongqing 400014, China Abstract: Objective To investigate the expressions of thyroid transcription factor-1 (TTF-1), the type II alveolar epithelial cells (AECII)-specific marker, and vimentin, the fibroblast-specific marker, in the lungs of neonatal mice with bronchopulmonary dysplasia (BPD) and explore the pathogenesis of BPD. Methods Neonatal mice were exposed to hyperoxia to induce BPD, and pathological changes in the lung tissues were examined. At 3, 7, 14 and 21 days after the exposure, the protein and mRNA expressions of TTF-1 and vimentin were detected by double-labeled immunofluorescence assay and real-time PCR, respectively. Results Compared wi