江西东乡普通野生稻耐冷qtl定位及芽期耐冷相关基因的候选-作物遗传育种专业论文.docxVIP

AbstractLow—temperature Abstract Low—temperature is of the most important factors affecting high-yield，stable yield and quality of rice．In this study,an advanced backcross population，derived from between Dongxiang Common wild rice(Oryza rufipogon Griff．)and Indica cultivar Guichao 2(Oryza sativa L．)，was used to identify QTLs for cold tolerance at booting to flowering stage．Meanwhile，one cold—tolerance introgression line(IL l 1 2)selected from the population was used to explore QTLs genes for cold tolerance at early seedling stage by integrated strategy combining microarray with QTL mapping． 1．Three QTLs for cold tolerance at booting to flowering stage were detected chromosome 1，6 and 1 1 using advanced backcross population(BC4F2)．At qRLT 1—1 and qRLT6—1，the alleles from Dongxiang common wild rice(DXCWR)could enhance the cold tolerance of the backcross progenies． 2．ILl 12，a cold tolerant introgression line，Was identified via evaluation for cold tolerance at the early seedling stage 284 introgression lines from the same population．All the seedlings of the ILl 1 2 line survived normally for 9 days at 4～5。C low temperature，while Guicha02 died after 5 days under same treatment．So secondary population(F2：3 population)for cold—tolerant QTL mapping Was developed from the backcross between GC2 and IL l 1 2．Seven cold—tolerance QTLs were detected chromosomes l，2，5，6，7 and l 0 at the early seedling stage，with contribution rates ．ranging from 8％20％by QTL analysis using the F2：3 population．A total of 3337 predicted genes were found from TIGR by scanning the chromosomal region covered these QTLs． 3．The expression profiles of IL l 1 2 and GC2 under low-temperature and control conditions were obtained by performing Affymetrix rice whole genome array hybridization．Eight main genes from DXCWR related to cold tolerance were identified by integrated strategy combining microarray with QTL mapping．And the results were confirmed by PCR amplification，DNA sequence and quantitative RT-PC