不同炮制方法对槐角黄酮类成分量的影响-天津中草药杂志社.PDF

中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 22 期 2014 年 11 月 ·3271 · 不同炮制方法对槐角黄酮类成分量的影响 * 都盼盼，石延榜，张振凌 ，沈莎莎 河南中医学院，河南 郑州 450003 摘 要：目的 比较槐角不同炮制品中黄酮类成分的量。方法 采用 HPLC 法，Zorbax Eclipse Plus C18 色谱柱（250 mm × 4.6 mm ，5 μm ）；柱温30 ℃；流动相为甲醇-0.4%冰乙酸水溶液，梯度洗脱；体积流量 1.0 mL/min；检测波长256 nm 。结果 染料木苷：生品（0.86% ）＞蜜炙（0.67% ）＞炒炭（0.48% ）；芦丁：生品（3.0% ）＞蜜炙（2.2% ）＞炒炭（0.88% ）；槐角 苷：生品（8.08% ）＞蜜炙（5.73% ）＞炒炭（3.58% ）；槲皮素：生品（0.04% ）＜蜜炙（0.05% ）＜炒炭（0.12% ）；染料木 素：生品（0.06% ）＜蜜炙（0.08% ）＜炒炭（0.21% ）；山柰素：生品（0.01% ）＜蜜炙（0.02% ）＜炒炭（0.54% ）。结论 槐 角饮片中黄酮类成分炮制后染料木苷等黄酮苷成分的量降低，苷元的量升高，可能是饮片功效不同的原因。 关键词：槐角；蜜槐角；槐角炭；染料木苷；芦丁；槐角苷；槲皮素；染料木素；山柰素 中图分类号：R283.1 文献标志码：A 文章编号：0253 - 2670(2014)22 - 3271 - 04 DOI: 10.7501/j.issn.0253-2670.2014.22.010 Effects of different processing methods on content of flavonoids from Sophorae Fructus DU Pan-pan, SHI Yan-bang, ZHANG Zhen-ling, SHEN Sha-sha Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China Abstract: Objective To compare the content of flavonoids from Sophorae Fructus by different processing methods. Methods HPLC method with Zorbax Eclipse Plus C18 column (250 mm × 4.6 mm, 5 μm) was used in the experiment; Methanol-0.4% acetic acid was used as mobile phase, with gradient elution; Column temperature was set as 30 ℃; The flow rate was 1.0 mL/min and detection wavelength was 256 nm. Results Genistin: crude (0.86%) stir-frying with honey (0.67%) carbonizing by stir-frying (0.48%); Rutin: crude (3.0%) stir-frying with honey (2.2%) carbonizing by stir-frying (0.88%); Sophoricoside: crude (8.08%) stir-frying with honey (5.73%) carbonizing by stir-frying (3.58%); Quercetin: crude (0.04%) stir-frying with honey (0.05%) carbonizing by stir-frying (0.12%); Genistein: crude (0.06%) stir-frying with honey (0.08%) carbonizing by stir-frying (0.21%); Kaempferide: crude (0.0