响应面法优化超声辅助提取车前草中的熊果酸.PDFVIP

8 0 2011, Vol. 32, No. 06 食品科学 ※工艺技术 响应面法优化超声辅助提取车前草中的熊果酸 孔 涛，范杰平* ，胡小芳，曹 婧，张 璐，童 声 (南昌大学环境与化学工程学院，江西南昌 33003 1) 摘 要：目的：优化超声辅助提取车前草中的熊果酸工艺。方法：在单因素试验基础上，采用响应面法，以熊 果酸的提取率为响应值，通过回归分析各工艺参数与响应值之间的关系，并由此预测最佳的工艺条件。结果：超 声提取的最佳条件为提取温度75 ℃、乙醇体积分数90% 、功率4 80W 、液固比5 :1(mL/g) 、提取时间2 5min ，该条 件下提取3 次，熊果酸的提取率达到0 . 14 4 % 。结论：响应面法优化超声辅助提取车前草中熊果酸的预测准确、方 便，所得的最佳提取工艺条件高效、可行。 关键词：响应面法；超声提取；车前草；熊果酸 Optimizzation of Ultrasound-assisted Extraction of Ursolic Acid from Plantago asiatica L. by Response Surface Methodology KONG Tao ，FAN Jie-p in g* ，HU X iao-fang ，CA O Jing ，ZHAN G Lu ，TON G Sh en g (School of Environmental and Chemical Engineering, Nanchang University, Nanchang 33003 1, China) Abstract ：Obj ective: To optimize the ultrasound-assisted extraction of ursolic acid from Plantago asiatica L.. Methods: On the basis of single-factor experiments, response surface methodology (RSM) was used to explore the optimal conditions for extracting ursolic acid from Plantago asiatica L. following regression analysis of the relationship between extraction parameters and extraction rate of ursolic acid. Results: The optimal extraction conditions were extraction temperature of 75 ℃, ethanol concentration of 90%, ultrasound power of 480 W, liquid/material ratio of 5:1, and extraction time of 25 min. Under the optimal extraction conditions, the extraction rate of ursolic acid was 0.144%. Conclusion: The optimal extraction conditions are reasonable and effective. Key words ：respon se surface methodology (RSM)；ultrasound-assisted extraction ；P lantag o asiatica L .；ursolic acid 中图分类号：TS20 1.1 文献标识码：A 文章编号：1002-6630(20 11)06-0080-05 车前草又名车前、车轮草、猪儿草、钱串草等， 1 材料与方法 为车前科植物(P lan