脱氧核酶在细菌研究领域的现状与展望.PDFVIP

. 487 . 1001-8689(2013)07-0487-05 1 2,* (1 637007 2 610500) DNA10-23 10-23 10-23 R378 A Status and prospect of deoxyribozyme in the research fi eld of bacterial 1 2 Wang Tong-hui and Ling Bao-dong (1 Institute of Materia Medica and Department of Pharmacology, North Sichuan Medical College, Nanchong 637000; 2 Institute of Materia Medica and Department of Pharmacology, Chengdu Medical College, Chengdu 610500) Abstract The single stranded DNA molecule with catalytical activity and the recognition ability, which is synthesized by molecular evolution technique in vitro, is called deoxyribozyme. 10-23 deoxyribozyme is one of the most widely used deoxyribozymes. It’s not only widely used in antitumor, antiviral, genetic disease and so on, but also attracted more and more attention in the research fi eld of bacterial resistance in recent years. This paper will introduce 10-23 deoxyribozyme mainly from several aspects, such as the structure and target characteristics, cutting principle, design standard, chemical modifi cation method and the research status in the research fi eld of bacteria. Key words Antisense technology; 10-23 deoxyribozyme; Gene silencing RNADNA (DNADNA enzyme) (ribozyme)(deoxyribozyme)1978 Zamecnik[1]13 10-23(10-23 DRz) DNA (systematic evolution of ligands by exponential enrichment SELEX) 1987Cech[2]RNA DNA (ribozymes)