香蕉MaUFGT1基因克隆及表达分析.DOC

香蕉MaUFGT1基因克隆及表达分析 胡会刚，吴桂文，胡玉林， 庞振才， 段雅婕，李伟明，孙德权， 谢江辉 基金项目：国家香蕉产业技术体系湛江试验站项目（CARS-31-16）；现代农业人才支撑计划 基金项目：国家香蕉产业技术体系湛江试验站项目（CARS-31-16）；现代农业人才支撑计划。 作者简介：胡会刚(1980一)，男，副研究员，博士，研究方向:热带果树生理与分子生物学。E—mail： HYPERLINK mailto:huhuigang@sina．com; huhuigang@sina．com; *通讯作者(Corresponding author)：谢江辉(XIE Jianghui)，E—mail：313972374@ 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室，广东湛江 524091 摘要：从香蕉果肉中克隆得到了一个类黄酮-3-O-葡糖基转移酶基因UFGT1基因全长，利用生物信息学方法分析了该基因的基本理化性质、蛋白二级结构、亚细胞定位、亲疏水性、信号肽、跨膜区、磷酸化位点、基因结构、保守结构域、系统进化关系，并对该基因的时空表达模式及香蕉果实不同发育阶段进行了表达分析。结果表明：UFGT1基因长1380bp，编码459个氨基酸的蛋白质；该蛋白为疏水性蛋白，亚细胞定位在叶绿体，蛋白无跨膜结构，不存在信号肽；MaUFGT1在不同组织中均有表达，但表达丰度存在差异；MaUFGT1在‘巴西’和‘香粉1号’两个不同品种中，随着果实成熟，其表达量逐渐上升，后期‘香粉1号’明显高于‘巴西’。这些结果表明，MaUFGT1可能参与了香蕉果肉黄酮类物质的合成，为进一步研究MaUFGT基因的功能奠定了基础。 关键词：香蕉；UFGT；基因克隆；生物信息学；表达分析。 中图分类号：S668.1 Cloning and Expression Analysis of Banana MaUFGT1 Gene Hu Huigang, Wu Guiwen, Hu Yulin, Pang Zhencai, Duan Yajie, Li Weiming, Sun dequan, Xie Jianghui* Institute of South Subtropical Corp Research, Chinese Academy of Tropical Agricultural Science/ Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091,China Abstract：In this study, a flavonoid-3-O-glucosyltransferase gene（UFGT） was cloned from bananas. The basic physicochemical properties, protein secondary structure, subcellular localization, hydrophobicity, signal peptide, transmembrane region, phosphorylation site, gene structure, conserved domain and phylogenetic relationship of the gene were analyzed by bioinformatics method. The expression pattern of the gene and the different developmental stages of the banana fruit were analyzed. The results showed that the UFGT gene was 1380 bp, which encoded 459 amino acids. The protein was hydrophobic and the subcellular was located in the chloroplast. Transmembrane structure and no signal peptide was not present in the protein. MaUFGT1 was expressed in different tissues, and the expression abundance was different. Expression of MaUFGT1 in the ‘BaXi’ and ‘xiangfen 1’banana gradually inc