《干细胞分离培养与纯化技术》相关实验技术-BCSCs enrichment.pdfVIP

THeMed ArTICLe y Emerging Molecular Diagnostic Technologies Review For reprint orders, please contact reprints@ Breast cancer stem cell enrichment and isolation by mammosphere culture and its potential diagnostic applications Expert Rev. Mol. Diagn. 13(1), 49–60 (2013) 1 Emerging knowledge about cancer stem cells (CSCs) is raising attention about the need to Katayoon Saadin and Ian M White*1,2 provide a more precise and complete diagnosis including the molecular profile of a patient’s 1 CSCs. As opposed to simply treating the bulk of the tumor, a more complete diagnosis can lead Chemical Physics Program, University of Maryland, College Park, MD 20742, to treatment regimens designed to eradicate CSCs from a patient. In this review the authors USA detail the application of the mammosphere assay in the study of breast CSCs. The authors then 2Fischell Department of Bioengineering, describe the potential transition of the mammosphere assay from the research laboratory to the University of Maryland, College Park, clinic by leveraging microsystems technology, which enables the integration of multiple functions MD 20742, USA *Author for correspondence: into a single automated device. To conclude the review, the authors project that future clinical Tel.: +1 301 405 6230 devices will be capable of isolating circulating metastatic cells from patient blood, enriching the ianwhite@ dangerous CSCs, and providing a molecular profile of the CSCs, thus arming physicians with