MERSCoV棘突蛋白主要特性及BT细胞抗原表位预测分析.docxVIP

MERS-CoV棘突蛋白主要特性及B/T细胞 抗原表位预测分析 于娟李红卢園園饶华祥雷有菊姜双应赵生仓 青海省疾病预防控制中心 摘要： 目的应用生物信息学方法分析屮东呼吸综合征冠状病毒(Middle East respiratory syndrome coronavirus, MERS-CoV)棘突蛋口 (Spike, S)的主要 特性，预测可能的B/T细胞抗原表位。结果MERS-CoV的S蛋白氨基酸序列基本 保守，具有多个糖基化、酰胺化及磷酸化翻译后修饰位点。综合各种单参数预测 MERS-CoV S蛋白可能存在4个B细胞抗原表位、7个CTL细胞表位和5个Th细 胞表位。方法以MERS-CoV S蛋白的氨基酸序列一级结构为基础分析其保守性， 采用ProtParam预测S蛋口的理化特性，S0PMA预测其二级结构，MotifScan预 测翻译后修饰位点，DNAStar分析其序列的亲水性指数、柔韧性指数、可及性参 数以及抗原指数，推测B细胞抗原表位的可能位置，采用SYFPEITHI的T细胞表 位预测工具预测CTL和Th细胞表位。结论 生物信息学方法预测MERS-CoV S蛋 白氨基酸保守序列，该蛋白可能含有B/T细胞抗原表位，为MERS-CoV疫苗研制 及其他相关研究奠定了基础。 关键词： MERS-CoV; Spike蛋白；B/T细胞表位;生物信息学; 作者简介：赵生仓，E-mail : 601877664@qq. com 作者简介：于娟(1983-)，女，山西长治人，硕士，副主任技师，主要从事呼 吸道病毒病原学检测。E-mai 1:yujuan2006008@163. com 收稿日期：2017-04-22 基金：国家〃十二五〃科技重大专项(No. 2012zxl0004-208) Characteristics and prediction of B-and T-cell epitopes of the Spike protein from MERS-CoV YU Juan LI Hong LU Nan-nan RAO Hua-xiang LEI You-ju JIANG Shuang-ying ZHAO Sheng-cang Qinghai Center for Disease Control and Prevention; Abstract： Objective To predict the main characteristics and B-and T-ccll epitopes of the Spike protein (S) of Middle East respiratory syndrome coronavirus (MERS-CoV) . Methods Analysis of the extent to which the S protein of MERS-CoV was conserved was performed based on its primary structure. Physical and chemical properties were predicted using ProtParam, and the secondary st rue turc of the proto in was predic ted usi ng SOPMA online software. Post-translational sites were predicted using the software MotifScan. B-cell epitopes were analyzed using the software DNAStar, which was used to analyze hydrophilicity, flexible regions, surface-accessible regions, and the antigenic index. T~cel 1 epi topes were analyzed using SYFPEITIIL RcsuIts The amino acid sequencc of the S protein from MERS-CoV was conserved, and mutations at 23 sites were eviclent in more than two MERS-CoV isolates. The protein had several posttranslational sites, in eluding 23N-glycosylation sites, 21N-myristoylation sites, and 40 phospho