中国林蛙皮肤胶原蛋白的提取及体外抗氧化活性探究.pdfVIP

第46卷第4期 应　 用　 化　 工 Vol.46 No.4 2017年4月 Applied Chemical Industry Apr.2017 中国林蛙皮肤胶原蛋白的提取及体外抗氧化活性研究 赵媛媛,王战勇,张晶,苏婷婷,李萍 (辽宁石油化工大学 化学化工与环境学部,辽宁抚顺　 113001) 摘　 要:以中国林蛙皮肤为实验原料,采用酶解法水解提取其胶原蛋白。 考察了提取次数、料液比、提取时间、提取 温度以及酶用量等因素对胶原蛋白提取效率的影响,确定中国林蛙皮肤胶原蛋白的提取条件为:胃蛋白酶提取,提 取次数2次,料液比1∶60 (g/ mL),提取时间36h,提取温度37 ℃,酶用量1∶100。 还进一步考察了林蛙皮肤胶原蛋 白的体外抗氧化能力。 结果显示,林蛙皮胶原蛋白具有较强的羟基自由基清除能力,但实验范围内其超氧自由基 和DPPH 自由基清除能力较弱。 关键词:林蛙;胶原蛋白;提取;抗氧化 中图分类号:TQ917　 　 文献标识码:A　 　 文章编号:1671-3206(2017)04-0685-04 Extraction of collagen from skin of Rana chensinensis and its antioxidant capacity in vitro ZHAO Yuan-yuan,WANGZhan-yong,ZHANGJing,SU Ting-ting,LI Ping (College of Chemistry,Chemical Engineering and Environmental Engineering, Liaoning Shihua University,Fushun 113001,China) Abstract:The collagensfromskinofRanachensinensiswereextractedusingenzymaticextractionmethod. The effect factorswhich include extraction frequency,ratio of liquid to raw material,extraction time,ex- traction temperature,andamountof enzymewerestudied,respectively.Theextractionconditionswerecho- sen as follows:enzyme is pepsin,extraction frequency is 2,water to raw material ratio is 60,extraction time is36h,extractiontemperatureis37 ℃ andamountof enzymeis1∶100.Invitroantioxidantactivities of the collagen from skin of Rana chensinensis were assayed.The results showed the collagenfrom skin of Rana chensinensis has strong scavenging ability of hydroxyl free radical,but the scavenging ability of su- peroxide anion free radical and DPPHfree radical isweak. Key words:Rana chensinensis;collagen;extraction;antioxidant 　 　 中国林蛙(Rana chensinensis)———黄蛤蟆,一直 较低,可降解,已将其广泛地应用在医学、滋养保健