正负交配型担孢子接种法鉴定重要甘蔗品种系对黑穗病的-甘蔗糖业.PDF

甘蔗糖业 2013 年第 1 期，2013 年2 月 Sugarcane and Canesugar No. 1, Feb. 2013 正负交配型担孢子接种法鉴定重要甘蔗品种(系)对黑穗病 的抗性 陈健文，沈万宽，杨湛端，陈仲华，刘 睿，陈勇生，邓海华* (广州甘蔗糖业研究所 广东省甘蔗改良与生物炼制重点实验室，广东广州510316) 摘 要：以正负交配型担孢子菌株组成的混合菌液作为接种体，对 30 个重要甘蔗品种( 系)进行浸泡接 种，鉴定其对黑穗病的抗性。结果表明，抗性水平为高抗(HR)的材料有 CP84-1198 、CP89-2143 等 9 份；抗性水平为抗(R)的材料有粤糖 99-66 、粤糖 06-581 和 Q190；抗性水平为中抗(MR)的有 HoCP93-746 、 ROC22 和 ROC24；CP63-588 、ROC25 和 NCo310 这 3 份材料表现为中感(MS)；CP72-1210 和 F134 等 9 份材料表现为感(S) ，而 CP85-384 、HoCP95-988 和粤糖 63-237 则高感(HS)黑穗病。此外，还初步推 断广东省湛江遂溪蔗区存在不同于台湾报导的小种 1、2 和 3 的甘蔗黑粉菌生理小种。 关键词：甘蔗；黑穗病；正负交配型；抗性鉴定 中图分类号：S566.1 文献标识码：A 文章编号：1005-9695(2013)01-0023-04 Evaluation of Some Important Sugarcane Varieties for Smut Resistance Using the Inoculation Method Basing on Plus and Minus Mating Type Sporidia CHEN Jian-wen, SHEN Wan-kuan, YANG Zhan-duan, CHEN Zhong-hua, LIU Rui, CHEN Yong-sheng, DENG Hai-hua (Guangzhou Sugarcane Industry Research Institue/Guangdong Key Lab of Sugarcane Improvement Biorefinery, Guangzhou 510316) Abstract: The resistance to the sugarcane smut of 30 important sugarcane clones was identified using the dipping inoculation method basing on plus and minus mating type sporidia. The results showed that CP84-1198, CP89-2143 and other 7 clones were highly resistant to smut (HR); Yuetang 99-66, Yuetang 06-581 and Q190 were resistant to smut (R); HoCP93-746, ROC22 and ROC24 were of the middle resistance to smut (MR); CP63-588, ROC25 and NCo310 were of the middle sensitivity to smut (MS); CP72-1210, F134 and other 7 clones were sensitive to smut (S); CP85-384, HoCP95-988 and Yuetang 63-237