荧光磁性表面分子印迹聚合物对柚皮苷的荧光偏振检测-中国管理科学.PDF

150 2017, Vol.38, No.18 食品科学 ※成分分析 荧光磁性表面分子印迹聚合物对柚皮苷的 荧光偏振检测 赵 晨，孟祥艳，陆文总，郭 星 （西安工业大学电子信息工程学院，陕西西安 710021 ） 摘  要：采用荧光磁性表面分子印迹法对柚皮苷进行荧光偏振检测。该方法以二氧化硅包裹的磁性颗粒为基质，甲 基丙烯酸和丙烯酰胺为单体，并用异硫氰酸荧光素标记，得到同时具有荧光和磁性的表面分子印迹聚合物。并采用 荧光偏振法和紫外分光光度法对制备的聚合物结合能力进行检测。通过与比较2 种检测方法可知荧光偏振法更为灵 敏，检出限为0.1 mg/L。最后对番茄酱中含有的柚皮苷进行回收率检测，回收率达到81.3% 以上。说明采用荧光磁 性表面分子印迹法对食品中柚皮苷可快速、高效地检测。 关键词：柚皮苷；表面分子印迹聚合物；磁纳米颗；荧光偏振；紫外分光光度计 Fluorescence Magnetic Surface Molecularly Imprinted Polymer Nanospheres to Detect Naringin by Fluorescence Polarization ZHAO Chen, MENG Xiangyan, LU Wenzong, GUO Xing ’ ’ (College of Electronic and Information Engineering, Xi an Technological University, Xi an 710021, China) Abstract: A fluorescent magnetic surface molecular imprinting method was used to detect narigin by fluorescence polarization technology. By using TiO2-coated magnetic particles as substrate and methlacrylic acid and acrylamide as monomers, a surface molecular imprinting polymer with both fluorescence and magnetic characteristics was prepared and loaded with fluorescein isothiocyanate. The binding ability of the prepared polymer was tested by fluorescence polarization and ultraviolet (UV) spectrophotometry. Compared two other methods, the fluorescence polarization method was more / sensitive, and its limit of detection (LOD) was 0.1 mg L. The recovery of the fluorescence polarization method was higher than 81.3%. It was shown that the fluorescent magnetic surface molecular imprinting technique could be a new method to