急性高原反应大鼠模型的建立及多指标综合评价.pdf

军 杂 201591409 716 [] 70 Wistar ()5000m6000m12 2448h 10Morris / Morris 5000m24h 6000m12h (P 0.05)48h / [] []R339.54 R322.35 []A []0577-7402(2015)09-0716-06 [DOI]10.11855/j.issn.0577-7402.2015.09.06 Reproduction of a rat model of acute high-altitude sickness and evaluation of its related indexes WANG Chi 1 2 1 2 1 2 1 , YAN Mu-yang , DUAN Jin-yan , CHEN Jing-wen , HUANG Jie , GUAN Xu , XIANG Dai-jun , JIANG 2 1 Hui , WANG Cheng-bin 1Department of Clinical Laboratory, 2Department of Hyperbaric Chamber, General Hospital of PLA, Beijing 100853, China * Corresponding authors. WANG Cheng-bin, E-mail: wangcb301@126.com; JKANG Hui, E-mail: jm7111@163.com [Abstract]ObjectiveTo reproduce a rat model of acute mountain sickness by using a hypobaric chamber to simulate the plateau environment, and to study the related physiological parameters. Methods A total of 70 male Wistar rats were randomly divided into normal control (NC) group, and 6 hypobaric hypoxia groups (10 each) in which rats were housed in a hypobaric chamber and exposed to the enviroment simulating high altitude of 5000m and 6000m for 12h, 24h and 48h respectively. Memory Morris water maze test was conducted to evaluate the changes in working memory of rats in space. Histological changes in lung and brain tissue were observed. Blood from abdominal aorta was collected to analyze the parameters of blood gas. The histopathologi cal changes in lung and cerebrum were observed, and their wet/dry ratios were calculated. Results Reduction in activity or even death was found in hypobaric hypoxia groups, and arterial PaO2 was significantly decreased in all rats of hypobaric hypoxia groups. Compared with NC group, rats exposed to a simulated altitu