芦荟大黄素与DNA相互作用的紫外光谱和电化学研究-分析测试学报.PDF

26 1 V ol126 N o1 1 2007 1 FEN X I CESH I XU EBAO ( Journal of Ins trum en tal A naly s s) 59~ 6 1 DNA 万红艳, 张亚锋, 陈敬华, 吴 萍, 郑艳洁, 林新华 ( , 350004) : pH ( A E) DNA , pH 414 , AE DNA , pH 615 714 , , - pH 714 , ( A k ) , s : ; DNA; ; : Q 523; O 657132; O6571 1 : A : 1004 - 4957 ( 2007) 0 1- 0059- 03 S tud es on the Interact on betw een A loe-em od n and DNA w th UV - V s Spectrom etry and Cycl c V oltamm etry WAN H ong-yan, ZHANG Y a-feng, CH EN J ng-hua, WU P ng, ZHENG Y an-j e, L IN X n-hua ( D epartm ent o f Pha rm aceut ca l, F uj an M ed ca lU n vers ty, Fuzhou 350004, Ch na) Ab stract: The pH e ffect on the nteract on betw een aloe-em od n(A E) and DNA w as stud ed by UV - V s spectrom etry and cycl c vo ltamm etry( CV ) . The resu lts o f the CV study show ed that the nteract on w as m a nly of an e lectrostat c one n phosphate buffer so lut on at pH 414, whereas ntercalat ve b nd ng played a dom nant ro le at pH 615 and 714 solut ons. The results of the UV - V s spectroscop c study further conf rm ed them. W th the k net c param eters, such as charge transfer coeff c ent A and standard e lectron transfer rate constant ks n the absence and presence of DNA for the calcu lat on, the ca lculated resu lts show ed that an e lectrochem cal act ve supram o lecu lar com p lex w as form ed bewt een A E and DNA. Key wo rds: A loe-em od n; DNA; UV - V s spectroscopy; Cycl c vo ltamm etry DNA ,