紫外-可见分光光度法测定生大黄和醋大黄的总蒽醌-井冈山大学学报.PDFVIP

第38 卷第6 期 Vol.38 No.6 井冈山大学学报( 自 科学版) 2017 年11 月 November 2017 Journal of Jinggangshan University (Natural Science) 88 88 井冈山大学学报( 自然科学版) 文章编号：1674-8085(20 17)06-0088-05 紫外-可见分光光度法测定生大黄和醋大黄的 总蒽醌含量 * 魏江存，陈 勇，谢 臻，李耀华，邓丽红，阙祖亮，庞丹青 （广西中医药大学药学院，广西，南宁 530020 ） 摘 要：目的 建立紫外分光光度法测定生大黄和醋大黄总蒽醌的含量。方法 以大黄素为对照品，浓度为1%的醋酸镁甲 醇溶液对其进行显色，在513 nm 波长处测定吸光度，测定生大黄和醋大黄总蒽醌的含量。结果 比较其含量差异，揭示大 黄炮制前后总蒽醌的含量变化。结论 该方法可作为大黄不同炮制品的质量控制方法。 关键词：生大黄；醋大黄；总蒽醌；紫外分光光度法 中图分类号：R284.2 文献标识码：A DOI:10.3969/j .issn.1674-8085.20 17.06.018 DETERMINATION OF TOTAL ANTHRAQUINONE CONTENTS IN RAW RHUBARB AND VINEGAR RHUBARB BY ULTRAVIOLET-VISIBLE SPECTROPHOTOMETRY * WEI Jiang-cun, CHEN Yong, XIE ZHen, LI Yao-hua, DENG Li-hong, QUE Zu-liang, PANG Dan-qing (College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Guangxi 530020, China) Abstract Objective: The method of ultraviolet-visible spectrophotometry for determining the contents of total anthraquinone in raw rhubarb and vinegar rhubarb was established. Methods: Using emodin as the reference substance, 1% magnesium acetate methanol solution for its colration, the absorbance was measured at the wavelength of 513 nm for determing the contents of total anthraquinone in Raw rhubarb. Results: Vinegar rhubarb, and the differences were compared for assure the change of the content of total anthraquinone before and after the processing of rhubarb. Conclusion:This method can be used as a quality control method for different processed products of rhubarb. Key words raw rhubarb; vinegar rhubarb; total anthraquinone; UV