邓恩桉叶挥发性成分的提取及分析田玉红南京林业大学学报.PDFVIP

邓恩桉叶挥发性成分的提取及分析 田玉红1,2，刘雄民1*，周永红1，黄宏妙1 (1．广西大学化学 『七工9院。广西 南宁 530004;2．广西．Y-学院生物与化学工程系，广西 柳州 545006) 摘 要 ：采用水蒸气蒸馏法从邓恩桉 叶中提取挥发油 中的油相成分，用乙醚作为溶剂从蒸馏残液中萃取挥发 油的水溶性物质 ，并利用气相色谱一质谱 (GC—MS)联用分析 比较二者的化学成分。邓思桉叶挥发油油相部分 的得油率为3．67％(以干重计)，其 中确认 了52个组分 。占油相部分总量 的97．04％，油相部分主要成分为 1，8一桉叶油素(24．71％)，其次为香树烯(9．10％)、γ-松油烯(8．87％)、α-蒎烯(7．67％)等；水溶性成分的得油 率为0．s5％(以于重计)，其 中确认 了36个组分 ， 占水溶性部分总量的83．52％，香树烯 (19.40％)和蓝桉醇 (15．67％)是水溶部分的主要成分。 关键词：邓恩桉；挥发油；水溶性成分 中图分类号：O657．7 文献标识码：A 文章编号：1000-2006(2006)02-0055-04 ExtractionandDeterminationofVolatileConstituentsofLeaves fromEucalyptusdunnii TlAN Yu-hong1,2 , LIU Xiong-min1*,ZHOU Yong-hong1,HUANGHong-miao1 (1.DepartmentofChemistryandChemicalEngineeringGuangxiUniversity,Nanning530004,China;2.Departmentof BiologicalandChemicalEngineeringG,angxiTechnologyUniversity,Liuzhou545006,China) Abstract:Thevolatileconstituentsofleaves/tomEucalyptusdunnii,includingoilfraction andwater-solublefraction,wereextractedanddetermined.Oilfractionofvolatilecompo- nentswasobtainedthroughsteamdistillation.Etherwasusedasasolventtoextractthewa- ter-solublefractionofthevolatilecompoundsfromtheleftliquidaftersteamdistillationin order toknow thequantityandconstituentsofvolatilecompoundsdissolved in thewater phase.Totheoilfraction,yieldofoilwas3.670//00 (dryweightbasis),andtothewater-soluble fraction,itwas0.85% (dryweightbasis).Bothoilfractionandwater-solublefractionwere analyzed by gas chromatography-mass spectrometry method. The results showed that 52 compoundsthatwere97.04% of theoilfractionwere identifiedand36compoundsthatwere 83.52%ofthewater-solublefractionwereidentified.Themajorconstituentsinoilfraction were 1,8-cineole (24.71%),followedbyaromadendrene (9.10%), γ-terpinene (8.87%), α- pinene (7.67%). Aromadendrene (19.40%)andglobulol (15.670//00)werefound tobethe majorcompoundsinthewater-solublefraction. Keywords:Eucalyptusdunnii;Volatileoil;Water-solublefraction