金水清对大鼠肾小球系膜细胞增殖及凋亡的影响-儿科-湖北中医院.PDFVIP

2005 12 6 12 # 691 # * ¹ ¹ ¹ 刘晓鹰 张雪荣 倪珠英 1 2 : 通过观察中药复方金水清对大鼠肾小球系膜细胞( mesangial cells, MC) 增 及凋亡的影响, 探讨 其治疗小儿血尿的作用机制: 以噻唑蓝法检测 MC 的增 , 荧光显微镜下观察MC 的凋亡形态, 流式细胞仪检 测细胞周期及凋亡: 在 1 000~ 62. 5 Lg/ L 浓度范围内, 金水清能抑制MC 增 , 可使 G ~ G 期细胞增高, 0 1 G - M期细胞减少, 且呈浓度依赖关系, 并能明显诱导 MC 凋亡: MC 是金水清发挥作用的重要靶细胞, 金水清 2 可能通过: ( 1) 减少细胞有丝分裂, 干扰细胞周期, 抑制系膜细胞的增 及代谢活性; (2) 诱导系膜细胞的凋亡等环节发 挥抑制MC 增 的作用, 从而减少炎性因子的合成与释放, 减少细胞外基质的聚积, 达到治疗小儿血尿, 延缓慢性肾脏 病理进程的目的 12 金水清口服液 血尿 肾小球系膜细胞 增 凋亡 Effect of Jinshuiqing on Proliferation and Apoptosis of Glomerular Mesangial Cells LI U Xi aoy ing, ZHAN G Xuerong, NI Zhuy ing H ubei H osp i tal of T CM , Wuhan (430061) ABSTRACT Ob ective: To study the effect of Jinshuiqing on proliferation and apoptosis of glomerular mesangial cells ( MC) and its mechanism in the treatment of hematuria in children. Methods: Proliferat ion of glomerular mesangial cells w as assessed by MTT assay. Fluorescent microscopy w as used to observe t he morphology of apoptosis and flow cytometry w as used to study cell cycle and apoptosis. Results:T he Jinshuiqing groups (ranged from 1 000~ 62. 5Lg/ L) inhibited the prolif- eration of MC. T he ratio of G ~ G cell increased and the ratio of , G - M cell decreased in a dose- dependent manner. 0 1 2 Jinshuiqing induced the apoptosis of mesangial cells. Conclusion: MC is the main target for action of Jinshuiqing . ( 1) It can re- duce mitosis and inhibit t he proliferation