寒地獭兔肠道菌群定植的初步研究-畜牧学；动物遗传育种与繁殖专业论文.docxVIP

CONTENTSAbstract CONTENTS Abstract in Chinese ． ． ． ． ． ． 。I Abstract in English ．．III 1．IntrOductiOn 1 1．1 Rex Rabbit 1 1．1．1 External morphology of Rex Rabbit 1 1．1．：!Diet habits of Rex Rabbit ． ． ． ． ． ．．1 1．1．3 Coprophagy of Rex Rabbit ．．1 1．1．4 Characteristics ofRex Rabbit digestive system．．．．． ．．． ．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．． ．．．．．．．．．．．2 1．1．5 The problem ofdiarrhea in Rex Rabbit ． ． 3 1．2 Progress of intestinal flora 4 1．2．1 The conception of intestinal flora ．4 1．2．2 The cIassjfication of intestinal flora ．4 1．2．3 Physiological role of intestinal flora 6 1。2．4 The imbalance of intestinal flora 。。 ． ． ． ． ．．7 1．2．5 The harmful of imbalance in intestinal flora 7 1．3 Research the colonization of gut flora ．．8 1．3．1 Conception ofcolonization ．．8 1．3．2 Colonization resistance ．．8 1．：}．3 The conditions of colonization 9 1．：；．4 The factors of influence colonization ．．9 1．4 Research progress analysis methods ofdiversity ofmicrobial flora．．． ．．．．．． ．．．．．．．．．．．．． 10 1．4．1 Traditional research methods 10 1．4．2 Real-Time PCR ．．1() 1．4．3 DGGE ．11 1．5 Objectives and significance of experimental research 11 2．Materials and Methods ． ． ． ． ． ． ．．1：1 2．1 Experimental materials ．1：1 2．1．1 Experimental animals ．．1 2 2．1．2 Preperation of soft excrement and intestinal fluid freeze—dried powder 12 2．1．3 Main reagents ．．13 2．1．4 Main instruments ． 。 ． 1：； 2．1．5 Primer design and synthesis． ． ． ．．14 2．2 Experimental methods ． ． ． ．．1zI 2．2．1 Extraction the genomic DNA of contents in gut of Rex Rabbit 14 2．2．2 Detection the content and purity ofgenomic DNA 15 万方数据 东北农业大学农学硕士学位论文2．2．3 东北农业大学农学硕士学位论文 2．2．3 Detection the specificity of primer pairs ．1 5 2．2．4 Quantitative detection ofbacteria 一15 2．2．5 Statistics analysis of experimental data 16 3．Results and Analysis ． ． ． ． ． ． 17 3．1 Conventional PCR reaction 17 ：；。2 Standard curve ． ．。 ． ． ． ． l 7 3．3 Melt curve ．．20 3．4 Quantitative detection ofbacteria in intestinal contents 20 3．4．1 Detection the c