海藻酸PEG半互穿网络干细胞扩增载体的制备与表征-材料学专业论文.docxVIP

ABSTRACT The typical W/O suspension system was used to form the alginate based microsphere carriers in this paper. Alginate was selected as the matrix material of crosslinked system, in addition, polyethylene glycol (PEG) were introduced into this system to construct the semi-interpenetrating network structure (Semi-IPNs).The alginate/PEG semi-IPNs microcarriers contained disulfide bonds within the structure, whose greatest characteristic was that disulfide bond cleavage can occur under the influence of a particular reducing agent. Microcarriers were characterized by infrared spectroscopy (FT-IR), SEM, particle size distribution statistics. Compared with microcarriers not with PEG, the precise degradation behaviors control of microcarriers herein prepared with semi-IPNS can be achieved. We achieved the controllability of the degradation characteristics and particle size of microcarriers by adjusting the molecular weight of PEG, the mass ratio of PEG and alginate and the type of the reducing agent. We also studied the modification time of chitosan and other impacts on micro-swelling properties and degradation time. Chitosan and heparin here were chosen to improve the cytocompatibility and mechanical properties through adsorbing to the surface of microcarriers. Furthermore, fibronectin (FN) and basic fibroblast growth factor (bFGF) were also immobilized onto the surface of microcarriers to facilitate human umbilical cord blood mesenchymal stem cells (hUCB MSCs) adhesion and proliferation. hUCB MSCs were cultured on the microcarriers prepared with different components. The cell morphology and proliferation conditions were studied here. Results showed that microcarriers prepared after modification can support cell adhesion and proliferation. Stem cells proliferated well on microcarriers modified with fibronectin indicating that the fibronectin can satisfactorily improve the biocompatibility of microcarriers. However, stem cells did not change significantly on microcarri