红树内生细菌AmS2对多种植物病原真菌的抑制作用-广东农业科学.PDF

广东农业科学 2013 年第 5 期 73 红树内生细菌AmS2 对多种植物病原真菌的抑制作用 2 1 2 1 欧雄常 柳 凤 何 红 昝丽梅 1.中国热带农业科学院南亚热带作物研究所袁 广东 湛江 524091曰 2.广东海洋大学农学院袁 广东 湛江 524088 冤 摘 要 采用平板对峙生长法测定了红树内生 菌AmS2 菌株对辣椒疫霉和香蕉枯萎菌等多种植物病原真菌的抑制作用袁通 过生长速率法研究了发酵液甲醇提取物对多种植物病原真菌的毒力作用遥 结果表明袁菌株AmS2 对多种病原菌均具有较强的抑制 作用袁其中对辣椒炭疽菌的抑制作用最强袁抑菌带宽为 11.5 mm 袁显微观察抑菌带边缘菌丝末端膨大成球状尧分枝增多尧 胞壁破 裂遥 发酵液甲醇提取物能够抑制 6 种病原真菌的生长袁随着浓度的增加袁抑菌效果增强袁其中对辣椒炭疽菌的抑菌效果最好袁EC50 和 EC95 分别为 0.8923 mg/mL 和 2.4939 mg/mL遥 关键词 红树曰 内生 菌曰 活性物质曰 毒力作用 + 中 分类号 S432.4 4 文献标识码 A 文章编号 1004-874X 2013冤05-0073-03 Antifungal activity of mangrove endophytic bacterium AmS2 against plant pathogenic fungi 1,2 1 2 1 OU Xiong-chang , LIU Feng , HE Hong , ZA N Li-mei 1.South A sia Tropical Crop Research Institute , Chinese A cademy of Tropical A gric ultural Sciences , Zhanj iang 524091, China; 2.College of A griculture, G uangdong Ocean Unive rs ity, Zhanj iang 524088, China冤 Abstract: Antifungal activity of endophytic bacterium AmS2 strain and its methanol extract against 6 pathogenic fungi of plant such as Phytophthora capsici and Fusarium oxys porum f.sp. c ube nse was tested in laboratory. The results showed that the strain AmS2 showed high antifungal activity against 6 different kinds of the pathogenic fungi, the hypha treated with strain AmS2 withered and deformed seriously. It was shown on PDA plates that the methanol extract had high inhibitory effect on the growth of the fungi, of whic