程序降温处理过程中Nutlin-3对含水生菜种子抗冷性的影响.pdfVIP

上海农业学报 2018,34(2):33-38 ActaAgriculturae Shanghai DOI:10.15955/j.issn1000-3924.2018.02.07 文章编号:1000-3924(2018)02-033-06 程序降温处理过程中Nutlin-3对含水生菜种子抗冷性的影响 ∗ 周 强,韩颖颖 ,沈梦琪,李维杰,刘宝林 (上海理工大学医疗器械与食品学院,生物系统热科学研究所,上海200082) 摘 要:以含水生菜种子为试验材料,通过添加外源动物泛素E3连接酶抑制剂(Nutlin-3),研究其对程序降 温过程中含水生菜种子抗冷性的影响。 结果表明:程序降温速率为60 ℃/h 的条件下,添加Nutlin-3会促进种子 的耐冻性,发芽率高于对照组,特别是在-20 ℃条件下最为明显。 说明程序降温过程中,泛素化调节参与了种 子的抗冷过程。 对泛素相关基因进行实时荧光定量PCR检测发现,在降温至-20 ℃时,Nutlin-3抑制了泛素相 关基因HOS1的表达水平,而促进了冷相关转录因子ICE1的表达水平,E3泛素连接酶基因COP1也受到Nutline- 3 的促进。 同时,Nutlin-3对SOD活性有影响,但是对脯氨酸含量无明显作用。 关键词:生菜;种子;程序降温;低温胁迫;Nutlin-3 中图分类号:S636.2;TS255.3 文献标识码:A Effects of Nutlin-3 on chilling resistance of hydrated lettuce seeds(Lactuca sativa) ∗ ZHOU Qiang,HAN Ying-ying ,SHEN Meng-qi,LI Wei-jie,LIU Bao-lin (School of Medical Instrument and Food Engineering,Institute of Biothermal Science and Technology, University of Shanghaifor Science and Technology,Shanghai200082,China) Abstract:In order to study the effects of Nutlin-3 on chilling resistance of hydrated Lactuca sativa seeds duringprogrammedcooling,theseedswereimbibedwithexogenousanimalubiquitinE3ligaseinhibitors (Nutlin- 3)before treated with programmed cooling.The results showed that Nutlin-3 enhanced the freezing tolerance of lettuce seedsaftercooling(withtherateof60 ℃/h).i.e,thegerminationrateof seedsimbibedwithNutlin-3was higher thanthatofthecontrolimbibitiongroup,whichwasthemostobviouswhenthetemperaturewasreducedto be-20 ℃.This result indicated that in the process of programmed cooling,the regulation of ubiquitination was involved in the freezing resistance of seeds. Real-time quantitative PCR analysis of ubiquitin-related genes indicatedthat Nutlin-