分散固相萃取-分光光度法联用测定粮谷中痕量镍.pdfVIP

80 粮食与油脂 2016 年第 29 卷第 11 期 分散固相萃取- 分光光度法联用测定粮谷中痕量镍 丁宗庆，吴承明，刘光东 （汉江师范学院生物化学与环境工程系，　湖北十堰　442000 ） 摘　要：研究了利用分散固相萃取– 分光光度法联用测定粮谷中痕量镍的方法，酚酞乙醇溶液加 入水中生成的微晶酚酞颗粒对Ni–DMG （丁二酮肟）配合物具有强吸附萃取能力，据此建立了测 定痕量镍的新方法。最佳试验条件下，方法的线性范围为3.0~380 μg/L （r 0.999 8 ），检出限为 1.2 μg/L 。方法已成功应用于粮谷样品分析，加标回收率在97.0%~102.0% 之间，相对标准偏差在 1.9%~3.1% 之间。 关键词：镍；分散固相萃取；分光光度法；微晶酚酞；粮谷 Dispersive solid phase extraction combined with spectrophotometric method for determination of trace nickel in grain DING Zong-qing ，WU Cheng-ming ，LIU Guang-dong （Department of Biology ，Chemistry and Environmental Engineering ，Hanjiang Normal University ，Shiyan 442000 ，Hubei ，China ） Abstract ：A novel method using dispersive solid phase extraction combined with spectrophotometry was established for the determination of trace nickel in grain . When phenolphthalein in ethanol solution was dripped into water ，the phenolphthalein would precipitate plenty of microcrystalline ， which had a strong ability of sorptive extraction to Ni–DMG complex. Accordingly ，a new method for determination of trace nickel was built. Under the optimum test conditions ，the linear range of the method was 3.0~380 μg/L （r 0.999 8 ），and the detection limits was 1.2 μg/L. The proposed method was successfully applied in the grain analysis. The recovery ranged in 97.0%~102.0%，and the relative standard deviations ranged in 1.9%~3.1%. Key words ：n ick el ；disp er siv e solid ph ase extr action ；sp ectr oph ot om etry ；micr ocry st allin e phenolphthalein ；grain 1008―9578（2016）11―0080―02 中图分类号：TS207.3 文献标识码：A 文章编号： 镍是人体必需的一种微量元素，是体内多种酶的 相萃取。由此建立了分散固相萃取