鲑鳟鱼皮、骨低聚活性胶原多肽的制备研究-植物学专业论文.docxVIP

Studies Studies on preparation and bioactivities of oligo--peptides of coil gen byby enzymaticenzymatic bondingbonding hydrolysishydrolysis technologytechnol02y from Trout skin and bone ABSTRACT The whole reseaeh study was aimed at preparative method of oligo-peptides of Mr3000Da of collagen which be easily absorbed by human body and easy to amplify by enzymatic bonding hydrolysis technology from skin and bone of cultivation Trout which live cold spring．Using biochemistry and enzyme engineering teehnologys，process of decolorization，Decalcification，optimization by quadrature，process control，purification et a1．have been done． The collagen peptides from Trout skin have been extracted by using papain and flavourzyme methods．Two kinds of enzyme were analyzed bonding with single factor test and orthogonal test method，at the same times，the way of bleaching and process improvement were tried．The results showed that the optimum addi— tion of papain was the ratio of material to liquid 1：22，the quantity of enzymethe O．6％，temprarure 60C，pH=6．4，the time of enzymehysis was 3 hours，and the yield of extraction of the collagen peptides from Trout skin was 1 4．33％．DH 1 0．33％．The optimum addition of flavourzyme was the ratio of material to liquid 1：20，the quantity of enzymethe 0．35％，temprarure 55℃，pH=6．5，the time of ymehysis was 2．5 hours，and the yield of extraction of the collagen peptides from Trout skin was 1 3．68％，DH 10．65％．Among three methods，using papain before and using flavourzyme after，which is the best method；Activated Carbon be used as decolorant．Finished product was all right after the process of colation， microfiltration，ultrafiltration，concentration，drying and cribration were done and purity coefficient was 91．04％，collagen peptides less than 2000Da were 82．23 ％．FARP value(3mg／mL)was 3．39mmol／L，efficiency of scavenging activity(3m g／mL)of hydroxy radical was 1 0．09％and efficiency of scavenging activity(3 mg／mL)of superoxide free radical was 3．95％． II Pepsin Pepsin and