海洋食品中生物胺和甲醛测定方法的研究-水产品加工及贮藏工程专业论文.docxVIP

(R20．998)。方法的精密度良好，达到RSD5．O％(n---6)。在不同海洋食品样 (R20．998)。方法的精密度良好，达到RSD5．O％(n---6)。在不同海洋食品样 品中，衍生法测定的平均回收率在83．9．89．6％范围内，RSD为3．8．6．8％Ca=6)， 满足了海洋食品中甲醛检测的要求。并应用该方法测定了海洋食品(鱿鱼丝、大 黄鱼、虾等)中的甲醛含量。本方法具有简便快速、灵敏可靠、适用性强等特点。 同时运用此方法初步探究了大鼠血液中甲醛的代谢情况。结果发现，腹腔注 射甲醛溶液后(200mg／kg大鼠体重)，5min内测定大鼠血液中甲醛的含量迅速 升高至最高点(10t-g／mL)，接着以较缓的速率降低直至大鼠死亡。该方法可以为 血液中微量甲醛的分析检测提供一种新的有效的分析手段。 关键词：海洋食品，生物胺，甲醛，固相微萃取技术，衍生 Study Study on the Determination of Biogenic Amines and FormIdehyde in Sea Food Abstract 1．An analytical method of determining biogenic f11]aines(aAs)of seafood with simple pretreatment of samples for a reverse-phase ion-pair I-IPLC analysis with post-column automatic o-phthalaldehyde(OPA)deriva血afion and fluorescence detection(FLD)was developed．The adraction ofbiogenic amines(coldperehlorie acid)，parameters ofHPLC(components ofmobile phase，gradient elution program and excitation and emission wavelengths of FLD)and post-column derivatization system(derivatization reaction reageats and derivatization temperature)were optimized to satisfy the needs ofbiogenie amines detection in sea foods．White prawn, squid and oyster were chosen to be analyzed．The linearity，repeatability and recovery of the method for seven an3ines(tyramine,putrescine,cadaverine，histamine, agmatine,spermidine and spermine)1】I佻evaluated．The internal standard (1，6-diaminohexane)was used for accurate determination of BAs．The method was linear for the am．ines studied at given concentrations很冬0．999)．The average recoveries ranged from 81．7％to 103．9％for all al血eS in these three s∞foods．The RSDs were less than 10％(1F18)．This optimized method was applied to detect biogenic alnines in white prawn,squid and oyster during refrigerated storage．Sensory analysis，pH value and total volatile base nitrogen(T-VBN)value were combined to evaluate the freshness quality ofthese law materials．The obtained results w91e to test the possibility of fmding useful indicators for the freshness of aquatic samples in quality control procedure of aquatic pro