官69断块采油菌种室内评价及矿场试验效果分析-微生物学专业论文.docxVIP

南开大学硕士学位研究生论文 南开大学硕士学位研究生论文 摘要 摘 要 微生物采油技术是目前国内外发展较迅速的三次采油技术之一。主要是 利用微生物降解、代谢有益物质等性能来降低原油粘度，降低油水界面张 力等，从而提高原油采收率。 本研究首先论述了微生物油藏活动条件及适合微生物采油的油藏筛选 标准，并对官69断块油藏基础进行研究，得出该断块基本适合微生物采油， 再根据官69断块开采现状、剩余油分布、油水井生产现状及注采关系，确 定了微生物注入井和受益井。接着针对官69断块油藏特点，对己有的菌种 DG002、80、N80进行室内评价、岩心物理模拟驱油实验及矿场培养基实验。 室内实验表明，上述菌种能够乳化原油，改善油品性质，产酸、产气，降低 油水界面张力，改善原油组分。发酵实验结果表明，DG002、80、N80菌在污 水配制的培养基中发酵繁殖旺盛。在有氧条件下、无氧条件下菌浓均增加， 表明菌种具有兼性生长性能。微生物驱油岩心物理模拟实验表明，N80在多 孔介质中可生长繁殖。在驱替压力下，复合菌N80随注入水运移，通过了多 孔介质。在模拟官69断块目前含水91％条件下，注入N80菌驱油可提高采收 率6．6％。最后对N80、1)6002、80菌种进行现场试验、动态监测及效果分析。 矿场试验表明，微生物驱油己见到了较好效果，明显减缓了产量递减趋势。 预计调整方案实施后，效果更佳。 关键词：嗜热微生物；菌种评价；物理模拟实验；效果评价 塑垄垄兰堡主堂堡堕窒生笙查 塑垄垄兰堡主堂堡堕窒生笙查 塑墨 Abstract The technique of microbial enhanced oil recovery(MEOR)is one of quicklv developing EOR technique in the wor]d today，which can enhance oil recovery by decreasing oil viscosity．decreasing oil—water interracial tension by microorganism degrading oil and metabolizing helpful product et a1． Firstly，the condition of microbial activity in reservoir and the reservoir selecting stand for MEOR are studied．The reservoir condition of G69 block were also studied．The results show that G69 block are basicallY fjtful for MEOR．Then the injection well and beneficial production well were chosen accordj ng to the current development feature of G69 block，residual oil distribution．the production actuality and the relation between the injection well and production well．Secondly，according to the reservoir feature of G69 block，we evaluated existing bacterium (N80，DG002，80)in laboratory，carried on physical simulatiOn experiments of oil displacement by microorganism in corn and did field culture medium experiments．The results of lab experiment show that the above bacterium can emulsify crude oil，improve the oil quality，produce sour，produce gas，lower the interface tension between oii and water，and improve crude oil constituent．The results of Ferment experiment indicate that the bacterium(N80，DG002．80)are eugonic in culture medium maked up by d