基质重建相关基因MXRA7在小鼠骨髓造血中的作用-动物学专业论文.docxVIP

内蒙古大学 内蒙古大学 硕士学位论文 THE ROLE OF M ATR IX REMODELLING ASSOCIATED GENE MXRA7 rN BONE MARROW HE～IATOPOIESIS 0F MICE AB STRACT The bone marrow microenvironment plays a very important role in marrow hematopoiesis，SO exploring the bone marrow microenvironment can know the regulation factors of bone marrow hematopoiesis accurately．In the bone marrow, the cells that in charge of hematopoiesis directly includes hemopoietic stem cells and progenitor cells，while other cells，such as BMSC，endothelial cells and fibroblast， maintain the hematopoietic microenvironment，secrete compl ex hematopoietic growth factor，express the adhesion molecules，secrete protein and polysaccharides to make up the extracellular matrix in order to ensure the smooth work of hemopoiesis． MXRA7 gene(Matrix Remodeling Associated 7)is one of the matrix reconstruction related genes．In the related research about the gene，it was found that there is a good a铲eement between the change of the gene and the dynamic change of the corneal neovascularization．In order to discuss the influence of the gene on the medullary hematopoiesis function，it has been studied the difference of the amount of red blood cell，granulocyte and blood cells in normal wild—type mice and KO mice The result shows that the amount of all kinds of blood cells reduce after the MXRA7 has been knocked out，while the amount of white blood cells and blood cells reduce 万方数据 基质重建相关基因MXRA7在小鼠骨髓造血中的作用 基质重建相关基因MXRA7在小鼠骨髓造血中的作用 尹娟娟 obviously when compared with red blood cells．When compared with the normal wild-type mice，the amount of blood cells of KO mice reduced by 20％，which differs a 10t from other cells．Then the difference of the bone marrow micro．environment of inlmune．mediated of two kinds of mice in vitro and vivo iS studied．And the differential expression of related hemopoiesic gene in bone marrow micro—environment is detected，such as G—CSF,M—CSF,Nestin，IL一6，SDF一1， Angptl3，TPO and SO on．It shows that hematopoietic related gene expression red