急性期脑梗死患者血清HO-1、UCB含量变化及临床意义-神经病学专业论文.docxVIP

摘要目的检测脑梗死患者急性期血清HO一1和UCB浓度的动态变化，探 摘要 目的检测脑梗死患者急性期血清HO一1和UCB浓度的动态变化，探 讨HO一1和UCB在脑缺血损伤中的临床意义。 方法序贯收集2003年8月．2004年2月在郴州市第一人民医院治疗 的发病24小时以内的符合试验入选标准的脑梗死患者36例，同时收 集相匹配的同期体检者30例做对照。分别用ELISA法及钒酸盐氧化 法测定脑梗死组发病第1、3、6天及对照组的空腹血清HO—l及血清 UCB浓度。入选病例在发病24小时内及发病72小时后行CT扫描 计算脑梗死体积。 结果 I脑梗死患者急性期血清HO—I浓度的动态交化 发病第1天HO．1浓度最高，与对照组比较有显著差异(pO．01)； 第3天和第6天HO．1浓度即迅速下降，发病后第3天HO．1浓度 显著低于第1天(po．01)，第3天与第6天相比无显著差异(po．05)； 第6天与对照组相比无显著差异性(p0．05)。 2脑梗死患者急性期血清UCB浓度动态变化 发病第1天脑梗死患者血清UCB浓度较对照组略为升高，差 异无显著性(PO．05)；第3天和第6天UCB浓度依次下降，发病 第3天较第1天UCB浓度下降有明显差异(pO．01)，发病第3天 UCB浓度与第6天相比无显著差异(PO．05)；发病第6天UCB浓 度显著低于对照组(pO．01)。 3脑梗死患者各检测指标间的相关关系 ①发病第1天脑梗死患者血清HO．1浓度和同期血清UCB浓度 呈正相关(FO．645，P0．05)。 ②脑梗死体积和发病第1天血清HO．1浓度呈正相关(F0．358， P0。05)。 ③脑梗死体积和发病第3天、第6天血清UCB浓度呈负相关 (r3=-0．335，P0．05；‘6=一0．267，P0．05)。 结论1 结论 1 HO一1的升高有可能是机体对脑缺血损伤的防御反应。 2 UCB可能在脑梗死的发病过程中具有一定的保护作用。 [关键词]脑梗死，血红素氧合酶．1，非结合胆红素 Ⅱ ABSTRACTOBJECTIVE ABSTRACT OBJECTIVE To study the changes of serum Heme oxygenase-1 (HO一1)and Unconjugated bilirubin(UCB)content in patients with cerebral infarcts，and speculate the effect of HO一1 and UCB in the cerebral ischemia injury． METHODS From the first hospital of Chenzhou 36 patients with acme cerebral infarcts that occurred within the first 24 hours were consecutively incorporated into the study between August 2003 and February 2004，and 30 healthy subjects were selected as control group．The change ofthe HO一1 in blood serum was detected by ELISA．At the sanle time the change of UCB in the blood serum were measured by Oxidation of Vanadate．These patients were observed at one day,third day，and sixth day after the onset．The subsequent volume of brain lesion as a consequence of stroke was measured by CT in 72 hours after the onset． RESUIJTS 1．The change ofriO一1 content in the blood serum The cogent of HO-1 reached the highest point in the first day after stroke onset，and then decreased gradually．HO一1 content within the first day after disease onset was higher than that in the control group f]P0．0 1)．There is no significant differe