己烯雌酚、雌二醇环境雌激素光度分析新方法分析-分析化学专业论文.docxVIP

聊城大学硕士学位论文 聊城大学硕士学位论文 ii ii 催化高碘酸钾猝灭罗丹明B荧光为检测体系，通过测定标记物中铁的含量来间接检测 己烯雌酚，建立了测定己烯雌酚的新方法。己烯雌酚的浓度在1.0 ng/mL～2000 ng/mL 范围内与荧光猝灭强度呈线性关系，检出限为0.66 ng/mL。该方法灵敏度高，选择性 好，线性范围较宽，将其应用到水样中己烯雌酚的检测，结果满意。 4、动力学荧光分析法测定己烯雌酚 基于在酸性介质中，己烯雌酚对羟自由基与吖啶橙氧化还原反应的阻抑作用， 建立了动力学荧光法测定己烯雌酚的新方法。该方法简单，快速，线性范围为 0.1～ 50 ng/mL，检出限为 0.03 ng/mL。将其用于鸡肉中己烯雌酚的测定，结果满意。 5、顺序注射动力学分光光度法测定痕量己烯雌酚 基于在酸性介质中，己烯雌酚对羟自由基与丁基罗丹明 B 氧化还原反应的阻抑 作用，结合顺序注射进样技术，建立了测定己烯雌酚的新方法。己烯雌酚在 5.0～100 ng/mL 和 100ng/mL~ 2.0 μg/mL 范围内与吸光度呈良好的线性关系，方法的检出限为 2.15 ng/mL。该方法简单，快速，将其应用于实际水样中的己烯雌酚检测，结果满意。 关键词：光度法；免疫分析；纳米材料；动力学荧光法；顺序注射；17β-雌二醇； 己烯雌酚 iii iii ABSTRACT The environment estrogen (also called Environmental Hormone, endocrine disruptors) refers the substance which exists in the environment, the substance can disturb the person or the animal secretion systerm with the in vivo estrogen (estrogen-like effect) or with the effect of rogen antagonism. Environmental Estrongen may enter into body of human or animals by food chain or directly contact and destroy normal level estrongen in blood. Sequentially, it had effect on procreation, upgrowth, tumor, nervous system, immunity system and so on. Kinds of estrongen consisted in environment. Not only they have influence on human healthy, but also threaten phyle. Low concentration of they can have more great effect on biology, too. Therefore, detecting methods of Environmental Estrongen have become main focus in international environmental chymistry and analytical chemistry. In this paper, new spectrophotometry methods for the determination of diethylstilbestrol and estradiol have been developed. The major content was described as follows: A novel immunoassay for the determination of diethylstilbestrol based on the fluorescence enhancement of Au nanoparticles A novel immunoassay method for the determination of diethylstilbestrol has been developed based on enhancing rhodamine6G fluorescence of Au nanoparticles (AuNPs) labeled goat anti-rabbit antibody. The immunoreaction is performed in a polystyrene micr