急性冠脉综合征患者循环内皮祖细胞数量与功能的变化-内科学(心血管病学)专业论文.docxVIP

浙江大学硕士学位论文Changes 浙江大学硕士学位论文 Changes in the number and activity of circulating endothelial progenitor cells in pafients with acute coronary syndrome Postgraduate Zhou Xinping Supervisor Pro．Chen Junzhu Department of Cardiovascular diseases College of Medicine Zhejiang University ABSTRACT Objective To investigate changes in the number and activity of circulating endothelial progenitor cells(EPCs)in patients with acute coronary syndrome(ACS)· Methods Total mononuclear cells(MNCs)were isolated by Ficoll density gradient centrifugation from patients with ACS(n=26)and matched control subjects(n_14)· Then the cells were plated on fibronectin-coated culture dishes．EPCs were identified as adherent cells double positive for DiLDL·uptake and lecfin binding by direct fluorescent staining under a laser scanning confocal microscope．EPCs were further documented by demonstrating the expression of VEGFR-2、ACl33 and CD34 with flow eytomeUy．EPCs proliferation WaS assayed by MTT aSsay．EPCs adhesion aSsay WaS performed by replating them Oil fibronectin-coated dishes，and then adherent cells were counted．IL．8、SDF．1 in the cell culture supematant of EPCs were determined by ELISA． Results The number of EPCs WaS significantly increased in subjects with ACS compared with that ofmatched control subjects(50．56-士10．45 VS 74．63士10．33 EPCs／ ×200 field．P=O．001)．In addition,the functional activities of EPCs such蠲 proliferative and adhesive capacity were impaired in patients with ACS．The release 2 浙江大学硕士学位论文of 浙江大学硕士学位论文 of IL·8．SDF—I protein into the cell culture supematant of EPCs was also diminished． Conclusion The present study demonstrated that the number of EPCs was increased while the functional activities of circulating EPCs such as proliferative， adhesive and secretive capacity Wfffe impaired in subjects with ACS． Keywords Coronary artery disease；Endothelial progenitor cells；Proliferation； Adhesion；IL-8：SDF·1 3 浙江火学硕．1：学位论文英文缩写与注解 浙江火学硕．1：学位论文 英文缩写与注解 abbreviation 缩写 全称 中文 ACS acute cor