不对称还原苯乙酮的酵母菌株筛选与分子鉴定-微生物学报.PDF

微生物学通报 DEC 20, 2008, 35(12): 1920~1925 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 不对称还原苯乙酮的酵母菌株筛选与分子鉴定 1 1,3 1 2 1 1* 朱冬眉 陈 丹 郑黎鹂 陈 华 杨志荣 孙 群 (1. 610064) (2. 610064) (3. 611731) 摘 要: 利用苯乙酮作为模式底物, 对 145 株菌株进行初筛和复筛, 获得一株具有高效立体选择 性的酵母菌株YS6-2, 能够不对称还原苯乙酮生成(S)-1-苯基乙醇。在苯乙酮浓度为70 mmol/L 时, 底物的初始转化率达 26.8%, 产物(S)-1-苯基乙醇的对映体过量值为 98.8%。基于形态学、生理生 化特征、18S rDNA 和26S rDNA D1/D2 区域的分析表明, YS6-2 为胶红酵母(Rhodotorula muci- laginosa) 。 关键词: 不对称还原, 苯乙酮, 酵母菌, 18S rDNA, 26S rDNA D1/D2, 系统发育 Screening and Molecular Identification of Yeast Strain for Asymmetric Reduction of Acetophenone ZHU Dong-Mei1 CHEN Dan1,3 ZHENG Li-Li1 CHEN Hua2 1 1* YANG Zhi-Rong SUN Qun (1. College of Life Sciences, Sichuan University, Chengdu 610064) (2. College of Chemistry, Sichuan University, Chengdu 610064) (3. Sichuan Maker Science Technology Co., Ltd , Chengdu 611731) Abstract: Yeast strain YS6-2 with high stereoselectivity was screened from 145 strains when the asymmet- ric reduction of acetophenone to (S)-1-phenethylalcohol was chosen as the model reaction. With the concen- tration of acetophenone at 70 mmol/L, YS6-2 yielded (S)-alcohol at 26.8% conversion and enantiomeric ex- cess reached up to 98.8%. Based on the analysis of 18S rDNA and 26S rDNA D1/D2 domain