褐蘑菇水溶性多糖分级组分的抗氧化性能-食品科学.PDFVIP

※基础研究 食品科学 2011, Vol. 32, No. 21 37 1 2 1 1 李芳亮 ，高 杨 ，刘 莹 ，王 锐 (1.辽宁工程技术大学理学院，辽宁 阜新 123000 ；2.辽宁省阜新市科技局，辽宁 阜新 123000) 采用水提醇沉法提取并精制褐蘑菇多糖(WPPA) ，用体积分数60%、70% 和 80% 乙醇分级醇沉分离得3 种 粗多糖 WPPA60 、WPPA70 和 WPPA80 ；通过还原力、清除超氧阴离子自由基(O－ 2 ·) 、清除羟自由基( ·OH)和 抑制 H 2 O2 诱导红细胞氧化溶血实验评价 3 种褐蘑菇多糖体外抗氧化能力。结果表明：WPPA60 、WPPA70 和 WPPA80 具有较强的还原能力，对 O－ 2 ·和·OH 具有较强的清除作用；对 H2 O2 诱导红细胞氧化溶血及 MDA 生 成有很强的抑制作用；抗氧化能力从强到弱依次是 WPPA70 ＞WPPA80 ＞WPPA60 。 还原力；褐蘑菇；多糖；抗氧化 Antioxidant Activity of Water-soluble Polysaccharide Fractions from Portabella Mushroom Agaricus bisporus 1 2 1 1 LI Fang-liang ，GAO Yang ，LIU Ying ，WANG Rui (1. College of Science, Liaoning Technical University, Fuxin 123000, China； 2. Fuxin Science and Technology Bureau, Fuxin 123000, China) Abstract ：Water extraction followed by fractional ethanol precipitation was used to purify water-soluble polysaccharides from portabella mushroom Agaricus bisporus (WPPA). The polysaccharide fractions WPPA60, WPPA70 and WPPA80 were obtained using three ethanol concentrations: 60%, 70% and 80%. Their antioxidant activity in vitro was assessed in terms of reducing power, scavenging activity against superoxide anion hydroxyl free radicals and inhibitory activity against H2O2-induced erythrocyte hemolysis. The results showed that WPPA60, WPPA70 and WPPA80 revealed strong reducing power, scavenging capacity against superoxide anion and hydroxyl free radicals and inhibitory effect on erythrocyte hemolysis and MDA generatio