基础研究Urinarymetabolomicsstudyofrenalcell-南方医科大学学报.PDF

J South Med Univ, 2015, 35(5): 763-766 doi 10.3969/j.issn. 1673-4254.2015.05.28 ·763 · 基础研究 基于气相色谱质谱技术的肾细胞癌患者尿液代谢组学分析基于气相色谱质谱技术的肾细胞癌患者尿液代谢组学分析 张 琳，李 玲，孔海瑞，曾方银 南方医科大学南方医院检验科，广东 广州 510515 摘要：目的对肾细胞癌（renal cell cancer, RCC ）患者的尿液进行代谢组学分析，建立数据模型并筛选特征代谢标志物。方法 采 用气相色谱质谱联用技术（gas chromatography mass spectrometry, GC-MS）分析27 例RCC 患者，26 例泌尿系其它肿瘤患者及 26 例健康人的尿液，利用SIMCA-P+ 软件进行主成分分析（principal component analysis, PCA ）及正交偏最小二乘法-判 别分析（orthogonal partial least-squares discriminant analysis,0PLS-DA），并筛选特征代谢产物。结果 构建了PCA（R2X=0.846 ， Q2=0.575）和OPLS-DA（R2X=0.736 ，R2Y=0.974 ，Q2Y=0.897）模型，筛选出14种差异代谢产物，主要是有机酸、马尿酸、色氨酸 及其降解产物，其中戊酸、丙二酸、戊二酸、己二酸、吲哚乙酸、氨基喹啉、喹啉及色氨酸在RCC 患者尿液中的含量显著高于正常 人（P0.01），同时RCC 组的尿液中戊酸、苯丙氨酸、6- 甲氧基-硝基喹啉的含量显著高于泌尿系其它肿瘤患者（P0.01）。结论 基于GC-MS 的代谢组学方法可以区分RCC 患者，筛选出的代谢产物可能是RCC 的诊断标志物，可进行深入研究。 关键词：肾细胞癌；代谢组学；GC-MS ；PCA ；OPLS-DA Urinary metabolomics study of renal cell carcinoma based on gas chromatography-mass spectrometry ZHANG Lin, LI Ling, KONG Hairui, ZENG Fangyin Laboratory Medicine Center, Southern Medical University, Nanfang Hospital, Guangzhou 510515, China Abstract: Objective To identify the biomarkers of renal cell cancer (RCC) through urine metabolic analysis. Methods Urine samples of 27 RCC patients, 26 patients with other urinary cancers and 26 healthy volunteers were examined with gas chromatography-mass spectrometry (GC-MS). SIMCA-P+ software was used for principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) to screen for the differential metabolites. Results PCA (R2X=0.846, Q2=0.575) and OPLS-DA (R2X=0.736, R2Y=0.974, Q2Y=0.897) model were established for the RCC patients and control subjects. Fourteen metabolites were selected as the characteristic metabolites, including pentanoic acid, malonic acid, glutaric acid, adipic acid, amino quinoline,