混合菌群合成聚羟基烷酸最佳工艺条件研究-市政工程专业论文.docxVIP

哈尔滨工业大学工学硕士学位论文 哈尔滨工业大学工学硕士学位论文 -II -II - Abstract Polyhydroxyalkanoates (PHA) is a type of promising thermoplastic which share identical properties with polypropylene. But with the advantage of being biodedradable. The traditional process for PHA production are based on pure cultures of particular microorganisms grown on well-defined media.However, its widespread application have been limited due to high production cost.The use of mixed culture from activated sludge may significantly reduce the production cost.The quantity of excess sludge generated from activated sludge process will reduce and be reused.therefore reducing the cost. On the other hand,The storage rate and production is lower compared with pure culture fermentation. Experiment have been done on how operational conditions of activated sludge process could enhance the PHA production capability of sludge.The operating conditions being investigated were the substrate concentration in influent, carbon-phosphorus ratio, carbon- nitrogen ratio, temperature and initial pH. The sludge was acclimated in the SBR reactor with balanced nutrition supplied acetate as the only substrate,The influence of substrate concentration on the synthesis of PHA is studied, in a certain range, PHA production increase with the carbon source concentration, beyond this range, PHA production no longer increased but show a downward trend.The best COD concentration for PHA synthesis is 800- 1000mg/L.The corresponding sludge loading is 1.6- 2kgCOD/kgMLSS?d; The largest production of PHA accounted for 20% of cell dry weight. PHA is stored as energy and carbon reserve material when microorganisms are supplied with unblanced nutrition.PHA production will be improved with limited nitrogen and phosphorus supply. The accumulation of PHA under two different conditions(nitrogen and phosphorus limitation) was studied in this research. The results showed that the activated sludge can accumulate PHA up to 59% of the sludge dry weight when carbo