Factor-1-GMP-(rHuIGF-1-GMP)课件.pdfVIP

Recombinant Human Insulin-like Growth Factor-1 GMP (rHuIGF-1 GMP) PrimeGene Technical Data Sheet Catalog Number: GMP-105-01 Source: Escherichia coli. Molecular Weight: Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. Quantity: 5µg/100µg/1mg AA Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA Purity: 98 % by SDSand HPLC analyses. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity 5 of 5.0 ×10 IU/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.0. Endotoxin: Less than 0.0 1 EU/g of rHuIGF-1 GMP as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0