甲胺磷胁迫下小白菜抗氧化机制研究-生物化学与分子生物学专业论文.docxVIP

⑧ ⑧ 硕士学位论文 ⅥASTER’S THESlS Abstract Plant cens can produce grate deaI of reactive oxygen species(ROS active oxygen species)eVen jn nomal physiolo百cal conditions．when the envim柚ent changes， such as heat，cold，drought，pesticide p0Ilution，the output of ROS will伊eatIy increase． The organelles of plant cell tllat generate ROS iIlvOlve chloroplast，mitochondrjon， peroxide eIlzyme，glyOxysome、endoplasmic reticulum，microsomal，cell membrane，cell wall and so on．ROS illdude 02‘、H202、．oH、H02’(hydyoperoxyl radical)、RO。(alkoxy Tadical)、ROO。(peroxy radical)、ROOH(hydroperoxide)、102(sin酉et oxygen)、HOx、 03柚d so on．ROS toxic to o昭anisms，which call damage no皿al cells’DNA protein aⅡd membranc Iesin．So the cens’ROS lcvels must be strictly contIoUed．The plant cells haVe ped-ect ROS-defense—system，which is composed of anti-oxygen enzyme and antioxidant． According t0 the study On the actiVe challges 0f Amti·oxygen enzyme such as sⅥpeToxide disInutase(soD)、catalase(CⅪ)、G蚣athione pe嬲id踞e(GPx)、CytochIome p450 of Chinese Cabbagc alld the chaIlges of the content of GSH under Metllamid叩hos aIId the total ROS and Anti—oxygen c印acity of Ch．mcsc cabbagc，several sensiIive biological indicators haVe been selected．Then the rcsponse mechanisms under Methamidophos s打ess is studied and some potential biochemical biomafkers of organic phOsphorus 0n ClliⅡesc ca由bage suggested f11nheL 1．The Anti-oxygen Capaci留of Chinese Cabbage Under Methamid— ophos In thjs papeL the actiVities of supefoxide dismutase(SOD)，catalase(CAT)， 百utathione pefoxidase(GPX)，glutathione(GSH)in Chinese cabbage were dete衄ined ill me following 7 days after sprayed by O．59，m2 Memamidophos．Thc fesults jndicated matthe actIvities of SOD，CAT and t}le content of GSH in Chinese cabbage wcre higher tkm that of the control in the 2-6 day’but the activities of GPX w器lower than the contr01． Whereafter the activmes Of GPX蛆d total a11d-Oxygen capacity have no significant chan鲈．At也e 7th day，all parame钯rs rcvived to址幢level of control saⅡlple．It suggc