鸡褪黑激素受体基因的多态性及其在不同组织中表达量的研究-动物遗传育种与繁殖专业论文.docxVIP

鸡褪黑激素受体基因的多态性及其在不同组织中表达量的研究 oviduct, ovary, brain and SCN and the difference between open antenatal and open postpartum. The results indicate that: after open antenatal, gene MTNR1A shows up-regulated expression in different tissues (except down-regulated expression in brain), the differences of expression level are not significant among different tissues at open antenatal and after open antenatal, but significantly different after open postpartum, and the level in ovary is the highest. Compared the open antenatal, after open postpartum, gene MTNR1B and MTNR1C showed down-regulated expression in every kind of tissue, except up-regulated expression in optic chiasma. In the same tissue, the expression level of gene MTNR1B are not significantly different between open antenatal and open postpartum, but the difference are significant in different tissues at open antenatal or open postpartum, moreover the level of optic chiasma is always the highest and lowest in ovary. However for gene MTNR1C, it don’t only show significant difference in different tissues at open antenatal or open postpartum, but also be significantly different among heart oviduct and tissues of brain after open antenatal. The results of this study may be helpful to investigate the mechanism of melatonin regulating chickens’ reproductive performance . Keywords: chicken, MTNR gene, polymorphisms, egg laying performance, association analysis 4 符号说明 5’-RACE 5′-UTR 5-HTPD AANAT AFMK AMK C3-OHM cAMP cDNA CT 值 E2 FSH GnRH GPR50 HIOMT HPG LD LH LHRH LOD MEL mRNA MTNR MTNR1A MTNR1B MTNR1C NE rapid amplification of 5′-cDNA ends 5′-untranslated region 5-hydroxy tryptophan decarboxylases Arylalkylamine N-acetyltransferase N-acetyl-N-formyl-5-methoxykynuramine N-acetyl-5-methoxykynuramine cyclic 3-hydroxgmelatonin complementary deoxyribonuclei acid complementary DNA cycle threshold value estradiol follicle-stimulating hormone gonadotropin-releasing hormone G protein receptor 50 hydroxyindole-o-methyltransferase hyp