黄斑蓝子鱼PPARs和HNF4α基因的克隆及环境盐度对其mRNA海洋生物学专业论文.docxVIP

Abstract Abstract PAGE PAGE IV Abstract Peroxisome proliferator-activated receptors (PPARs) belong to the nuclear receptor family of ligand-activated transcription factors. PPAR activators can modulate various metabolic pathways in vivo, especially in lipid metabolism. There are three PPAR subtypes, named PPARα, PPARβ and PPARγ, encoded by different genes. Researches on the cloning and tissue-specific expressions of PPARs have been reported in many species of fish. However, studies on the function of PPARs, especially in the metabolism of high-unsaturated fatty acid (HUFA) in fish were seldom reported. Hepatocyte nuclear factor 4α (HNF4α) is a member of hepatocyte nuclear factor (HNF) family, which plays a key role in the regulation of differentiation of liver cells, and also participates in regulating expressions of specific genes involved in glucose and lipid metabolism in liver. HNF4α cDNA has been cloned in several fish species such as Danio rerio, Oreochromis niloticus and Sparus auratus. However, its function, especially in the metabolism of HUFA in fish is scarcely reported either. It is generally thought that marine fish are presumed to have a low capability or inability to synthesize HUFA. However, our previous study proved for the first time in a marine herbivorous teleost Siganus canaliculatus had the ability to synthetize HUFA. In order to further study the molecular regulatory mechanisms of HUFA biosynthesis in S. canaliculatus, genes encoding the key transcription factors PPARs and HNF4α were cloned in S. canaliculatus. Their tissue-specific expressions and the effects of environmental salinity on their expressions and fatty acid composition were also investigated, to probe their potential function in HUFA synthesis regulation. The results were shown as follows: Using RT-PCR and RACE-PCR, cDNAs of S. canaliculatus PPARs and HNF4α were obtained: PPARα is 2577bp in full length and encoding a polypeptide of 477 amino acids; PPARβ is 2584bp in full leng