环磷酰胺预处理、短程环孢素A联合骨髓腔内输注供者骨髓诱导大白兔特异性免疫耐受的研究-内科学专业论文.docxVIP

±1．47天)明显延长(PO．01)。 (2)供者骨髓细胞输注与供、受者融合后骨 髓细胞输注比较，移植皮肤生存期无显著性差异(PO．05)。(3)耐受诱导20 天时，第四、第五组受者对供者淋巴细胞的单向混合淋巴细胞反应较移植前 特异性降低，A490值分别为(0．41±0．063)、(0．45±0．019)，移植前为(1．44±0．041)，对无关第三者的反应性为(1．35±O．680)，移植后与移植前及无 关第三者相比较，差异均有显著性(Po．01)，移植前与无关第三者比较无 显著差异(PO．05)。【结论】l、在大白兔动物模型中，采用环磷酰胺预处理、短程环孢素A免疫 抑和输注供者骨髓细胞能诱导供受者造血嵌合体形成。2、骨髓细胞直接输注或供受者骨髓融合细胞输注均能获得较稳定的 混合嵌合体，延长移植皮肤生存时间，诱导供者特异性免疫耐受。3、供者骨髓细胞输注与皮肤移植同时进行能获得良好的免疫耐受。4、骨髓腔内输注骨髓细胞并发症少、植入率高。【关键词】骨髓移植；嵌合体；特异性免疫耐受；细胞融合；大白兔。Inducti。n。fdonor-typechimerismandtransplantati。n tolerance in non—myeloablatiVe rabbit by intra-bone。—marrowapplication ofinjection bone marrow cells and short-coursecyclosporine A．StudenttYang Xinping TutorChin Guo—An[objective]T。investigate the effect and mechanism8。f‘脚8plant— ati。n t。1erance induced by“b。ne marrow cell transplantati∞+cyc。lp— h。s。hamide(cy)’，pr。t。c。l c。mbined with sh。rt—c。ur88 applica‘1。n。‘cyclosporine A．，rMeth。ds]Thirty—six male New zealand rabbits and thirty-six±。mal。rabbits were used as d。n。rs and recipients respectiV。1y，mat。hlngwere diVidedpairs rand。mly．The thirty—six pair rabbits 1n‘C|6；r。：：；rand。mly： blank contr。1 group， simple pre-treated g删p，slmp上e b～llgr。up，experimental gr。up 1(cY+CSA+BMT)，exp8rimen‘a1 grou：third party d。n。r e。ntr。12(CY+CsA+￡usedb。ne marr。w cell infusion)an(1gruup·．S鲫edaySkingrafting wasperformed。nthed∞舱m∈：imwcells each was 3—4×108／k毋transplantation．The numbel^。fbone marrowthe third party sklnthe survival time。f skin graft，rejecti。n againstand mixed 1ymph。cyte reacti。nwere。bserved-The polym。ra8。∞ai．nthe嘞cti。n(PCR)was eⅢployed f。r the detecti。n。f SRY t。c。mfim[Results]The survival time of grafted skin was signific矾thprolonged．in the gr。up 4(44．67±3．20)and gr。up5(39·67±1·21)a8。。II：par：：：with the gr。up 1(10．83±o．75)，group 2(16·33±1·21)’gr。up 3。11’．?：+O．82)，andgr。up6(14．83±1．47)，respectively(P0_01)·MLRpr。11f。ratl。。1dem。nstrate the d。n。r specific inhibiti。n。f lymph。。yt8in the group 4 and group 5 were induced．In the group 4 and group 5， chimerism can be detected after 3